| Description: |
Typhaneoside is possibly the main active component in the bioactive fraction of Shaofu Zhuyu decoction, Shaofu Zhuyu decoction may have direct inhibitory effects on the contractility of the mice uterus. It has an inhibitory effect on the proliferation of human umbilical arterial smooth muscle cells(HUASMC) with a wide range of effective dose but the effect is started slowly. |
| In vitro: |
| Journal of Beijing University of Traditional Chinese Medicine, 2006, 29(5):307-10. | | Influence of typhaneoside on proliferation of human umbilical arterial smooth muscle cells.[Reference: WebLink] | To study the influence of Typhaneoside on the proliferation of human umbilical arterial smooth muscle cells(HUASMC).
METHODS AND RESULTS:
① HUASMC were primary cultured with tissue-sticking method and determined by α-actin detection.The third to fourth generations of cells were put into the experiment.② The highest concentration of Typhaneoside was 1.00×10-5 mol/L and then diluted in multiple proportions into 11 concentration groups,and a blank control group was established.After the stimulations were given to different groups for 24,48 and 72 hours,the toxicity of Typhaneoside were detected with MTT assay.③ The highest concentration of Typhaneoside was 1.00×10-6 mol/L and then diluted in 1/4 isobaric proportion into 6 concentration groups,and a blank control group was established. After the stimulations were given to different groups for 24,48 and 72 hours,the influence of Typhaneoside on the proliferation of HUASMC was observed with MTT assay. ① The cellular staining was positive under the microscope.② Typhaneoside was not toxic to HUASMC in the concentration ranges from 9.77×10-9 mol/L to 1.00×10-5 mol/L after 24,48 and 72 hours.③ Typhaneoside did not inhibit the proliferation of HUASMC in the concentration ranges of 9.77×10-10 mol/L to 1.00×10-6 mol/L after 24 or 48 hours,but inhibited significantly the proliferation of HUASMC after 72 hours in the concentration ranges of 1.56×10-8 mol/L to 2.5×10-7 mol/L.The effect was increased along with the dose increase but there was no significant difference.The inhibitory rate reached 9.91% to 14.99%.
CONCLUSIONS:
Typhaneoside has an inhibitory effect on the proliferation of HUASMC with a wide range of effective dose but the effect is started slowly. |
|
| In vivo: |
| Am J Chin Med. 2010;38(4):777-87. | | Inhibitory effects of active fraction and its main components of Shaofu Zhuyu decoction on uterus contraction.[Pubmed: 20626062] | Shaofu Zhuyu decoction is a famous formula for treating primary dysmenorrhea in China since the Qing dynasty. In this paper, the inhibitory effects of active-guided fraction and its main bioactive components of Shaofu Zhuyu decoction on a model of non-pregnant mice uterine contraction induced by oxytocin in vitro were investigated.
METHODS AND RESULTS:
Qualitative and quantitative chemical analyses were used to correlate the chemical composition of active fraction with the spasmolytic effects. Seven ingredients in the active fraction were identified and quantified by HPLC-DAD. Three ingredients, ferulic acid, vanillic acid, and typhaneoside, were evaluated for their effects on mice isolated uterine contraction induced by oxytocin in vitro. The ED(50) of them were 63.0 microg/ml, 57.6 microg/ml, 109.7 microg/ml, respectively. Furthermore, the inhibitory activity of the combination of these three compounds was prior to the fraction and seven compounds group. The ED(50) was 65.5 microg/ml.
CONCLUSIONS:
The data stated that ferulic acid, vanillic acid, and typhaneoside were possibly the main active components in the bioactive fraction of Shaofu Zhuyu decoction. The study also implied that Shaofu Zhuyu decoction may have direct inhibitory effects on the contractility of the mice uterus and justified the traditional use of the prescription for treating the uterine cramping associated dysmenorrhea. |
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