| In vitro: |
| Chemistry of Natural Compounds, 2011, 46(6):902-5. | | Antituberculosis activity of glycosides from Stevia rebaudiana and hybrid compounds of steviolbioside and pyridinecarboxylic acid hydrazides[Reference: WebLink] | | Stevioside and steviolbioside, glycosides from Stevia rebaudiana Bertoni, in addition to hybrid compounds synthesized from steviolbioside and the antituberculosis drug isoniazid and its isomer nicotinic acid hydrazide exhibited moderate antituberculosis activity against M. tuberculosis strain H37RV in vitro (MIC = 7.5, 3.75, 5, and 10 μg/mL, respectively). | | Food Chem. 2016 Apr 1;196:155-60. | | Production of a bioactive sweetener steviolbioside via specific hydrolyzing ester linkage of stevioside with a β-galactosidase.[Pubmed: 26593477] |
METHODS AND RESULTS:
A β-galactosidase from Kluyveromyces lactis was found to specifically catalyze hydrolysis of the glycosyl ester linkage of stevioside to yield Steviolbioside, a rare sweetener that also exists in Stevia rebaudiana leaves.
In a packed bed reactor, a reaction coupling separation was realized and a production yield of Steviolbioside reached 90% in 6 h. The hydrolysis product Steviolbioside presented higher cytoxicity on human normal cells (hepatocytes cell L02 and intestinal epithelial cell T84) than stevioside did. Comparing to the typical chemotherapy agent, 5-fluorouracil (5-FU), Steviolbioside presents much lower cytotoxicity on all assayed human normal cells; it presented notable inhibition on human hepatocarcinoma cell Hep3B, human breast cancer cell MDA-MB-231 and human pancreatic cancer cell BxPC-3.
CONCLUSIONS:
The remarkable inhibition on MDA-MB-231 cells makes Steviolbioside a potential remedy for human breast cancer, when Steviolbioside is served as a natural sweetener. |
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