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  • 原花青素B2

    Procyanidin B2

    原花青素B2
    产品编号 CFN99558
    CAS编号 29106-49-8
    分子式 = 分子量 C30H26O12 = 578.52
    产品纯度 >=98%
    物理属性 Powder
    化合物类型 Flavonoids
    植物来源 The fruits of Vitis vinifera L.
    ChemFaces的产品在影响因子大于5的优秀和顶级科学期刊中被引用
    提供自定义包装
    产品名称 产品编号 CAS编号 包装 QQ客服
    原花青素B2 CFN99558 29106-49-8 10mg QQ客服:1457312923
    原花青素B2 CFN99558 29106-49-8 20mg QQ客服:1457312923
    原花青素B2 CFN99558 29106-49-8 50mg QQ客服:1457312923
    原花青素B2 CFN99558 29106-49-8 100mg QQ客服:1457312923
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    ChemFaces的产品在许多优秀和顶级科学期刊中被引用

    Cell. 2018 Jan 11;172(1-2):249-261.e12.
    doi: 10.1016/j.cell.2017.12.019.
    IF=36.216(2019)

    PMID: 29328914

    Cell Metab. 2020 Mar 3;31(3):534-548.e5.
    doi: 10.1016/j.cmet.2020.01.002.
    IF=22.415(2019)

    PMID: 32004475

    Mol Cell. 2017 Nov 16;68(4):673-685.e6.
    doi: 10.1016/j.molcel.2017.10.022.
    IF=14.548(2019)

    PMID: 29149595

    ACS Nano. 2018 Apr 24;12(4): 3385-3396.
    doi: 10.1021/acsnano.7b08969.
    IF=13.903(2019)

    PMID: 29553709

    Nature Plants. 2016 Dec 22;3: 16206.
    doi: 10.1038/nplants.2016.205.
    IF=13.297(2019)

    PMID: 28005066

    Sci Adv. 2018 Oct 24;4(10): eaat6994.
    doi: 10.1126/sciadv.aat6994.
    IF=12.804(2019)

    PMID: 30417089
    我们的产品现已经出口到下面的研究机构与大学,并且还在增涨
  • Heidelberg University (Germany)
  • Universidade de Franca (Brazil)
  • Mahatma Gandhi University (India)
  • Centrum Menselijke Erfelijkheid (Belgium)
  • University of Beira Interior (Portugal)
  • Chiang Mai University (Thailand)
  • Complutense University of Madrid (Spain)
  • Melbourne University (Australia)
  • Aveiro University (Portugal)
  • Kamphaengphet Rajabhat University (Thailand)
  • University of Brasilia (Brazil)
  • Deutsches Krebsforschungszentrum (Germany)
  • Universite de Lille1 (France)
  • Srinakharinwirot University (Thailand)
  • More...
  • 国外学术期刊发表的引用ChemFaces产品的部分文献
  • Molecules.2018, 23(7):E1659
  • Indian J Pharm Sci.2022, 84(4): 874-882.
  • Cell Physiol Biochem.2017, 43(4):1425-1435
  • Neurochem Int.2020, 133:104629
  • Fitoterapia.2021, 153:104995.
  • J Cell Physiol.2021, 236(3):1950-1966.
  • Planta Medica International2022, 9(01):e108-e115.
  • Food Funct.2022, D1FO03838A.
  • J Ethnopharmacol.2019, 244:112074
  • Molecules.2019, 24(6):E1177
  • Korean J. Medicinal Crop Sci.2021, 29(6):425-433
  • J Am Soc Mass Spectrom.2021, 32(5):1205-1214.
  • Antioxidants (Basel).2021, 10(9):1487.
  • Journal of Apiculture2019, 34(2):131-136
  • Molecules.2019, 24(24):E4536
  • Acta Chromatographica2021, 00960.
  • J Appl Biol Chem.2022, 65(4):pp.463-469.
  • J Mass Spectrom.2022, 57(2):e4810.
  • Front Neurosci.2019, 13:1091
  • Molecules.2021, 26(18):5665.
  • J Ethnopharmacol.2019, 241:112025
  • Industrial Crops and Products2019, 140:111612
  • J. of Agricultural Science2015, 1916-9760
  • ...
  • 生物活性
    Description: Procyanidin B2 has vascular protective, anti-diabetic nephropathy,anti-cancer, anti-inflammatory, and antioxidant activities. Procyanidin B2 inhibited NLRP3 inflammasome activation via suppression of AP-1 pathway, and up-regulated the expression of GSTP1 via a mechanism that involves ERK and p38 MAPK activation and Nrf2 translocation. It has anti- and pro-oxidant effects on metal-mediated DNA damage by interacting with H2O2 and metal ions.
    Targets: Caspase | ROS | AP-1 | IL Receptor | COX | NOS | p65 | NO | NF-kB | Nrf2 | ERK | JNK | p38MAPK
    In vitro:
    Chem Biol Interact. 2015 May 25;233:122-38.
    Mechanisms of DNA methyltransferase-inhibitor interactions: Procyanidin B2 shows new promise for therapeutic intervention of cancer.[Pubmed: 25839702]
    DNA methyltransferases (DNMTs) is a key epigenetic enzyme for pharmacological manipulation and is employed in cancer reprogramming. During past few years multiple strategies have been implemented to excavate epigenetic compounds targeting DNMTs.
    METHODS AND RESULTS:
    In light of the emerging concept of chemoinformatics, molecular docking and simulation studies have been employed to accelerate the development of DNMT inhibitors. Among the DNMT inhibitors known till date, epigallocathechin-3-gallate (EGCG) was identified to be effective in reducing DNMT activity. However, the broad spectrum of EGCG to other diseases and variable target enzymes offers some limitations. In view of this, 32 EGCG analogues were screened at S-Adnosyl-L-homocysteine (SAH) binding pocket of DNMTs and procyanidin B2-3, 3'-di-O-gallate (procyanidin B2) was obtained as potent inhibitor having medicinally relevant chemical space. Further, in vitro analysis demonstrates the efficiency of procyanidin B2 in attenuating DNMT activity at IC50 of 6.88±0.647 μM and subsequently enhancing the expression of DNMT target genes, E-cadherin, Maspin and BRCA1.
    CONCLUSIONS:
    Moreover, the toxic property of procyanidin B2 towards triple negative breast cancer cells to normal cells offers platform for pre-clinical trial and an insight to the treatment of cancer.
    Mol Nutr Food Res. 2015 Feb;59(2):262-9.
    Procyanidin B2 inhibits inflammasome-mediated IL-1β production in lipopolysaccharide-stimulated macrophages.[Pubmed: 25379992]
    Macrophage stimulation with bacterial LPS triggers inflammasome activation, resulting in pro-inflammatory IL-1β cytokine maturation and secretion. IL-1β underlies the pathologies of many diseases, including type-2 diabetes. Thus, the modulation of the inflammatory response through bioactive food compounds, such as procyanidins, is a powerful tool to promote homeostasis.
    METHODS AND RESULTS:
    To determine the role of procyanidin B2 in inflammasome activation, LPS-primed THP-1-macrophages were supplemented with or without procyanidin B2 . Western blot analysis of COX2 , iNOS, p65, NLRP3 and IL-1β was performed followed by p65 supershift assay, in vivo caspase-1 activation assay and NO, IL-1β and IL-6 determination. Procyanidin B2 mediated inhibition of inflammasome activation includes the inactivation of the NF-κB signalling pathway, the first stage required for the transcription of inflammasome precursors, through the inhibition of p65 nuclear expression and DNA binding, resulting in the transcriptional repression of target genes, such as COX2 , iNOS and production of IL-6 and NO. Furthermore, procyanidin B2 decreases NLRP3 and pro-IL-1β cytoplasmic pools, limiting components of inflammasome activation and impeding inflammasome assembly and caspase-1 activation, and finally secretion of active IL-1β.
    CONCLUSIONS:
    This study provides the first evidence that procyanidin B2 inhibits inflammasome activation and IL-1β secretion during LPS-induced acute inflammation in human macrophages.
    Free Radic Biol Med. 2005 Oct 15;39(8):1041-9.
    Procyanidin B2 has anti- and pro-oxidant effects on metal-mediated DNA damage.[Pubmed: 16198231 ]
    Procyanidin B2 (epicatechin-(4beta-8)-epicatechin), which is present in grape seeds, apples, and cacao beans, has antioxidant properties. We investigated the mechanism of preventive action of procyanidin B2 against oxidative DNA damage in human cultured cells and isolated DNA.
    METHODS AND RESULTS:
    Procyanidin B2 inhibited the formation of 8-oxo-7,8-dihydro-2'-deoxyguanosine (8-oxodG) in the human leukemia cell line HL-60 treated with an H2O2-generating system. In contrast, a high concentration of procyanidin B2 increased the formation of 8-oxodG in HL-60 cells. Experiments with calf thymus DNA also revealed that procyanidin B2 decreased 8-oxodG formation by Fe(II)/H2O2, whereas procyanidin B2 induced DNA damage in the presence of Cu(II), and H2O2 extensively enhanced it. An electron spin resonance spin trapping study utilizing 3,3,5,5-tetramethyl-1-pyrroline-N-oxide (M4PO) demonstrated that procyanidin B2 decreased the signal of M4PO-OH from H2O2 and Fe(II), whereas procyanidin B2 enhanced the signal from H2O2 and Cu(II). As an antioxidant mechanism, UV-visible spectroscopy showed that procyanidin B2 chelated Fe(II) at equivalent concentrations. As a pro-oxidant property, we examined DNA damage induced by procyanidin B2, using 32P-labeled DNA fragments obtained from genes relevant to human cancer.
    CONCLUSIONS:
    Our results raise the possibility that procyanidin B2 exerts both antioxidant and pro-oxidant properties by interacting with H2O2 and metal ions.
    In vivo:
    Chem Biol Interact. 2014 Sep 6;222C:68-76.
    Effect of cinnamon and its procyanidin-B2 enriched fraction on diabetic nephropathy in rats.[Pubmed: 25199697]
    Non-enzymatic protein glycation and resultant accumulation of advanced glycation endproducts (AGE) are implicated in the pathogenesis of diabetic complications including diabetic nephropathy (DN). It is considered that antiglycating agents offer protection against AGE mediated pathologies including DN. Earlier we characterized procyanidin-B2 (PCB2) as the active component from cinnamon (Cinnamomum zeylanicum) that inhibits AGE formation in vitro.
    METHODS AND RESULTS:
    In this study, we have investigated the potential of PCB2-enriched fraction of cinnamon to prevent in vivo accumulation of AGE and to ameliorate renal changes in diabetic rats. Streptozotocin-induced diabetic rats were fed with either 3% cinnamon or 0.002% PCB2-fraction in diet for 12weeks. Biochemical analysis of blood and urine was performed at the end of experiment. Evaluation of glomerular markers that serve as indicators of renal function was done by immunohistochemistry, immunoblotting and qRT-PCR. Supplementation of diabetic rats with cinnamon and PCB2-fraction prevented glycation mediated RBC-IgG cross-links and HbA1c accumulation in diabetes rats. Cinnamon and PCB2-fraction also inhibited the accumulation of N-carboxy methyl lysine (CML), a prominent AGE in diabetic kidney. Interestingly, cinnamon and its PCB2-fraction prevented the AGE mediated loss of expression of glomerular podocyte proteins; nephrin and podocin. Inhibition of AGE by cinnamon and PCB2-fraction ameliorated the diabetes mediated renal malfunction in rats as evidenced by reduced urinary albumin and creatinine.
    CONCLUSIONS:
    In conclusion, PCB2 from cinnamon inhibited AGE accumulation in diabetic rat kidney and ameliorated AGE mediated pathogenesis of DN.
    制备储备液(仅供参考)
    1 mg 5 mg 10 mg 20 mg 25 mg
    1 mM 1.7285 mL 8.6427 mL 17.2855 mL 34.571 mL 43.2137 mL
    5 mM 0.3457 mL 1.7285 mL 3.4571 mL 6.9142 mL 8.6427 mL
    10 mM 0.1729 mL 0.8643 mL 1.7285 mL 3.4571 mL 4.3214 mL
    50 mM 0.0346 mL 0.1729 mL 0.3457 mL 0.6914 mL 0.8643 mL
    100 mM 0.0173 mL 0.0864 mL 0.1729 mL 0.3457 mL 0.4321 mL
    * Note: If you are in the process of experiment, it's need to make the dilution ratios of the samples. The dilution data of the sheet for your reference. Normally, it's can get a better solubility within lower of Concentrations.
    部分图片展示
    产品名称 产品编号 CAS编号 分子式 = 分子量 位单 联系QQ
    原花青素; Proanthocyanidins CFN99556 4852-22-6 C30H26O13 = 594.52 20mg QQ客服:2159513211
    原花青素B2; Procyanidin B2 CFN99558 29106-49-8 C30H26O12 = 578.52 20mg QQ客服:1457312923
    原花青素B1; Procyanidin B1 CFN99557 20315-25-7 C30H26O12 = 578.52 20mg QQ客服:3257982914
    原花青素B3; Procyanidin B3 CFN99559 23567-23-9 C30H26O12 = 578.52 10mg QQ客服:1413575084
    原花青素B4; Procyanidin B4 CFN91171 29106-51-2 C30H26O12 = 578.5 10mg QQ客服:1457312923
    Afzelechin-(4alpha->8)-epiafzelechin; Afzelechin-(4alpha->8)-epiafzelechin CFN89432 1383627-30-2 C30H26O10 = 546.52 5mg QQ客服:3257982914
    Cinchonain IIb; Cinchonain IIb CFN89461 85022-68-0 C39H32O15 = 740.66 5mg QQ客服:215959384
    Cinchonain IIa; Cinchonain IIa CFN89439 85081-23-8 C39H32O15 = 740.66 5mg QQ客服:1413575084
    原花青素C1; Procyanidin C1 CFN99560 37064-30-5 C45H38O18 = 866.77 10mg QQ客服:1413575084
    Daphnodorin B; Daphnodorin B CFN92959 95733-02-1 C30H22O10 = 542.49 5mg QQ客服:3257982914

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