| Description: |
Ganoderol A has significant anti-inflammatory activity and protection against UVA damage. it has an inhibitory effect on angiotensin converting enzyme activity and cholesterol biosynthesis.
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| In vitro: |
| Trop. J. Pharm. Res., 2015, 14(3):412-21. | | In vitro Protective Effect of Ganoderol A Isolated from Ganadermalucidum Against Ultraviolet A Radiation and its Anti-inflammatory Properties[Reference: WebLink] | Purpose: To evaluate the ultraviolet A (UVA) protection and anti-inflammatory activity of ganoderol A extracted from Ganodermalucidum.
METHODS AND RESULTS:
Methods: The cytotoxicity and in vitro protective effect of ganoderol A against UVA damage were evaluated by MTT assay. Apoptosis and cell-cycle arrest of NIH/3T3 fibroblast cells were assayed by fluorescence-activated cell sorting (FCS). Expression of monocyte chemotactic protein-1 (MCP-1) and inducible nitric oxide synthase (iNOS) were determined using quantitative real-time polymerase chain reaction (qPCR). Results: The results indicate that the maximal non-toxic concentration of ganoderol A in NIH/3T3 cells and RAW 264.7 macrophages was 50 and 25 μg/mL respectively. DNA in the tails and tail length decreased by 55 and 70%, respectively, in the group pretreated with ganoderol A compared with the UVA-treated group. G1 phase cells decreased by 23%, whereas the number of apoptotic cells returned to normal. The expression of MCP-1 and iNOS declined to 60 and 15%, respectively, compared with LPS-stimulated group.
CONCLUSIONS:
Conclusion: Ganoderol A has significant anti-inflammatory activity and protection against UVA damage, thus suggesting that the compound is a candidate for the development of a suitable product to protect skin from UV-induced photoaging. |
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| In vivo: |
| Appl Environ Microbiol. 2005 Jul;71(7):3653-8. | | Effect of 26-oxygenosterols from Ganoderma lucidum and their activity as cholesterol synthesis inhibitors.[Pubmed: 16000773 ] | Ganoderma lucidum is a medicinal fungus belonging to the Polyporaceae family which has long been known in Japan as Reishi and has been used extensively in traditional Chinese medicine.
METHODS AND RESULTS:
We report the isolation and identification of the 26-oxygenosterols ganoderol A, ganoderol B, ganoderal A, and ganoderic acid Y and their biological effects on cholesterol synthesis in a human hepatic cell line in vitro. We also investigated the site of inhibition in the cholesterol synthesis pathway. We found that these oxygenated sterols from G. lucidum inhibited cholesterol biosynthesis via conversion of acetate or mevalonate as a precursor of cholesterol. By incorporation of 24,25-dihydro-[24,25-3H2]lanosterol and [3-3H]lathosterol in the presence of ganoderol A, we determined that the point of inhibition of cholesterol synthesis is between lanosterol and lathosterol.
CONCLUSIONS:
These results demonstrate that the lanosterol 14alpha-demethylase, which converts 24,25-dihydrolanosterol to cholesterol, can be inhibited by the 26-oxygenosterols from G. lucidum. These 26-oxygenosterols could lead to novel therapeutic agents that lower blood cholesterol. |
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