| Description: |
Ganoderic acid A , a representative active triterpenoid from Ganoderma lucidum, exhibits antinociceptive, antioxidative, cytotoxic, hepatoprotective and anticancer activities. It has a wide spectrum of targets including nuclear transcription factor-kappaB,activator protein-1,STAT,IL-6,JAK, and p38MAPK. |
| In vitro: |
| Nan Fang Yi Ke Da Xue Xue Bao. 2015 May 20;35(5):619-24. | | Ganoderic acid A suppresses proliferation and invasion and induces apoptosis in human osteosarcoma cells.[Pubmed: 26018252] | To investigate the effect of ganoderic acid A (GA-A) on the biological behaviors of human osteosarcoma cells in vitro.
METHODS AND RESULTS:
MG63 and HOS cells were treated with 0.1, 0.25, and 0.5 mmol/L GA-A, and the changes in cell proliferation, apoptosis and migration were evaluated using MTT assay, flow cytometry, and Transwell assay, respectively. The expressions of STAT3, p38, and NF-κB1 in the cells were analyzed by Western blotting.
GA-A effectively inhibited the proliferation of human osteosarcoma HOS and MG-63 cells in a dose-dependent manner, and induced obvious cell apoptosis in both cells. Treatment with 0.5 mmol/L GA-A also resulted in significant inhibition of the invasion of both cells. The results of Western blotting showed that GA-A down-regulated the expression level of phosphorylated STAT3 and increased the phosphorylation level of p38 and NF-κB1 expression in both cells.
CONCLUSIONS:
GA-A can induce proliferation inhibition, apoptosis and suppression of invasion in human osteosarcoma HOS and MG-63 cells. | | Int J Med Mushrooms. 2013;15(2):175-82. | | Production of Ginkgo leaf-shaped basidiocarps of the Lingzhi or Reishi medicinal mushroom Ganoderma lucidum (higher Basidiomycetes), containing high levels of α- and β-D-glucan and ganoderic acid A.[Pubmed: 23557369 ] |
METHODS AND RESULTS:
Ganoderic acid A and α- and β-D-glucan content were compared among morphologically different basidiocarps of the medicinal mushroom Ganoderma lucidum. Ginkgo leaf-shaped basidiocarps gradually hardened from the base to the pileus and accumulated a higher amount of bioactive components than normal (kidney-shaped) and antler/deer horn-shaped basidiocarps. In the normal G. lucidum stipe, the outer context contained the highest amount of α- and β-D-glucan (approximately 55%) and the highest amount of ganoderic acid A (approximately 0.3%).
CONCLUSIONS:
Ginkgo leaf-shaped G. lucidum had a large area of outer layer and stout outer context, which contributed to their high α- and β-D-glucan and ganoderic acid A content. |
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