| Description: |
Phloretin may potentially be an immunomodulator by impairing the activation and function of DCs, it is effective in preventing estrogen deficiency-induced osteoclastogenic resorption. Phloretin has antimicrobial activity against the Gram negative bacteria Salmonella typhimurium ATCC 13311. It has a wide spectrum of targets including ASK-1-MAPK, Smac, capase. |
| Targets: |
NF-kB | Bcl-2/Bax | Caspase | JNK | p38MAPK | ERK | ASK | TLR | ROS | gp120/CD4 | IFN-γ |
| In vitro: |
| Food Chem. 2014 Oct 1;160:292-7. | | Biochemical and antimicrobial activity of phloretin and its glycosilated derivatives present in apple and kumquat.[Pubmed: 24799241] | Phloretin and its glycosylated derivatives (phlorizin and phloretin 3',5'-di-C-glucoside) are dihydrochalcones that have many interesting biological properties.
METHODS AND RESULTS:
The results obtained showed that the dihydrochalcones are able to inhibit growth of Gram positive bacteria, in particular Staphylococcus aureus ATCC 6538, Listeria monocytogenes ATCC 13932 and methicillin-resistant S. aureus clinical strains. Moreover, phloretin is active also against the Gram negative bacteria Salmonella typhimurium ATCC 13311.
CONCLUSIONS:
The determination of the enzymatic activity of key metabolic enzymes allowed us to shed some light on the biochemical mechanism of aglycon cell growth inhibition, showing as it remarkably influences the energetic metabolism of S. aureus. In addition, structure/activity determinations highlighted that the presence of a glycosyl moiety bound to the chalcone structure dramatically decreases the antimicrobial activity of phloretin. |
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| In vivo: |
| Phytomedicine. 2014 Sep 15;21(10):1208-15. | | Phloretin promotes osteoclast apoptosis in murine macrophages and inhibits estrogen deficiency-induced osteoporosis in mice. [Pubmed: 24932975] | Bone-remodeling imbalance induced by increased osteoclast formation and bone resorption is known to cause skeletal diseases such as osteoporosis. The reduction of estrogen levels at menopause is one of the strongest risk factors developing postmenopausal osteoporosis.
METHODS AND RESULTS:
This study investigated osteoprotective effects of the dihydrochalcone phloretin found in apple tree leaves on bone loss in ovariectomized (OVX) C57BL/6 female mice as a model for postmenopausal osteoporosis. OVX demoted bone mineral density (BMD) of mouse femurs, reduced serum 17β-estradiol level and enhanced serum receptor activator of NF-κB ligand (RANKL)/osteoprotegerin ratio with uterine atrophy. Oral administration of 10 mg/kg phloretin to OVX mice for 8 weeks improved such effects, compared to sham-operated mice. Phloretin attenuated TRAP activity and cellular expression of β3 integrin and carbonic anhydrase II augmented in femoral bone tissues of OVX mice. This study further examined that osteogenic activity of phloretin in RANKL-differentiated Raw 264.7 macrophages into mature osteoclasts. Phloretin at 1-20 μM stimulated Smac expression and capase-3 activation concurrently with nuclear fragmentation of multi-nucleated osteoclasts, indicating that this compound promoted osteoclast apoptosis. Consistently, phloretin enhanced bcl-2 induction but diminished bax expression. Furthermore, phloretin activated ASK-1-diverged JNK and p38 MAPK signaling pathways in mature osteoclasts, whereas it dose-dependently inhibited the RANKL-stimulated activation of ERK.
CONCLUSIONS:
Therefore, phloretin manipulated ASK-1-MAPK signal transduction leading to transcription of apoptotic genes. Phloretin was effective in preventing estrogen deficiency-induced osteoclastogenic resorption. |
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