| Description: |
Neritaloside is a cardiac glycoside. Neritaloside exhibits central nervous system depressant activity in mice at a dose of 25 mg/kg. |
| Targets: |
Sodium Channel | ATPase | Potassium Channel |
| In vitro: |
| Anal Chem. 2000 Aug 1;72(15):3547-52. | | LC/MS/MS analyses of an oleander extract for cancer treatment.[Pubmed: 10952541] |
METHODS AND RESULTS:
An HPLC/MS/MS method has been developed for the characterization and quantification of the cardiac glycosides oleandrin, odoroside, neritaloside and the aglycone oleandrigenin, all contained in a patented-hot-water extract of Nerium oleander L (Anvirzel). Qualitative analysis of such extracts was achieved using a hybrid tandem quadrupole time-of-flight (QqTOF) mass spectrometer. Collision-induced dissociation (CID) mass spectra of oleandrin, oleandrigenin, odoroside, and neritaloside were obtained with greater than 5 ppm mass accuracy and resolution routinely in excess of 8000 (fwhm). The detection limit for oleandrin of 20 pg (injected) was realized when the precursor-to-product ion transition, m/z 577 --> 373, was monitored.
CONCLUSIONS:
We have also applied the analytical method to the determination of oleandrin, oleandrigenin, neritaloside, and odoroside in human plasma following an intramuscular injection of Anvirzel. |
|
| In vivo: |
| Phytochemistry. 1999 Feb;50(3):435-8. | | Bio-active cardenolides from the leaves of Nerium oleander.[Pubmed: 9933955] |
METHODS AND RESULTS:
A bioactivity directed isolation of the methanolic extract of the fresh, uncrushed leaves of Nerium oleander showing a central nervous system (CNS) depressant effect in mice has been undertaken. As a result, four CNS depressant cardenolides including a new cardenolide, neridiginoside and three known constituents, nerizoside, neritaloside and odoroside-H, have been isolated which exhibited CNS depressant activity in mice at a dose of 25 mg/kg.
CONCLUSIONS:
The structure of neridiginoside was elucidated as 3 beta-O-(D-diginosyl)-5 beta, 14 beta-dihydroxy-card-20(22)-enolide, using spectroscopic methods including one-dimensional and two-dimensional NMR (COSY-45, NOESY, J-resolved, HMQC and HMBC).
The known compounds have been indentified through spectral studies and comparison of data with those reported in the literature. |
|
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Mol Cell. 2017 Nov 16;68(4):673-685.e6. doi: 10.1016/j.molcel.2017.10.022.IF=14.548(2019)
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Sci Adv. 2018 Oct 24;4(10): eaat6994. doi: 10.1126/sciadv.aat6994.IF=12.804(2019)
