The uptake and transepithelial transport of the three main constituents macrocarpal A (M-A), Macrocarpal B (M-B), and cypellocarpa C (Cy-C) from the fruits of Eucalyptus globulus Labill. were investigated.
METHODS AND RESULTS:
Monolayers of the human intestinal epithelial cancer cell line Caco-2 were incubated with M-A, Macrocarpal B, and Cy-C to model its intestinal absorption and transport, respectively. The determination of compounds was performed by HPLC. The apparent permeability coefficients (P(app)) for M-A, Macrocarpal B, and Cy-C in the apical-to-basolateral direction of a Caco-2 monolayer were (1.70+/-0.06)x10(-6), (1.99+/-0.10)x10(-6), and (6.08+/-0.41)x10(-6)cm/s, respectively. In the presence of iodoacetamide, the P(app) of Cy-C were both reducted in apical-to-basolateral and basolateral-to-apical directions. M-A and Macrocarpal B appear to accumulate in the epithelial cells.
CONCLUSIONS:
The intestinal absorption of M-A, Macrocarpal B, and Cy-C was passive diffusion as the dominating process and Cy-C was partly ATP-dependent. |
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