| In vitro: |
| Molecules. 2012 Jun 13;17(6):7284-93. | | Antibacterial, antifungal and cytotoxic activities of two flavonoids from Retama raetam flowers.[Pubmed: 22695233] |
METHODS AND RESULTS:
The compounds licoflavone C (1) and Derrone (2) were active against Pseudomonas aeruginosa and Escherichia coli (7.81-15.62 μg/mL) and showed important antifungal activity. Strong antifungal activity against Candida species (7.81 μg/mL) was for example found with Derrone. The tested compounds also showed strong cytotoxicity against Hep-2 cells.
CONCLUSIONS:
Derrone may be interesting antimicrobial agents to be used against infectious diseases caused by many pathogens. | | Biol Pharm Bull. 2016 Jun 1;39(6):935-45. | | In Vitro Characterization of Derrone as an Aurora Kinase Inhibitor.[Pubmed: 26983907 ] | Among mitotic kinases, Aurora kinases are the most widely studied, since their expression is restricted to mitosis. They play a key role in chromosome segregation and cell polyploidy. Aurora kinases are important therapeutic targets, and several research groups have directed their efforts toward the identification of kinase inhibitors. The aim of this study is to screen and characterize Aurora kinase inhibitors from natural substances extracted from plants that are used in the Vietnamese pharmacopoeia.
METHODS AND RESULTS:
We have characterized in vitro Derrone, extracted from Erythrina orientalis L. MURR, as a novel Aurora kinase inhibitor. This compound exhibited an ability to inhibit the phosphorylation of histone H3 at ser10 both in kinase assay and at the cellular level. The compound was more effective against Aurora kinase B, with a lower IC50 value as compared to Aurora A. Moreover, it impaired the mitotic spindle checkpoint and led to endoreduplication in cancer cells, a phenomenon caused by an Aurora B inhibitor.
CONCLUSIONS:
Interestingly, using the xCelligence system and real-time cell analysis (RTCA) software, we set up a comparison of cell proliferation profiles between cancer cells treated with Derrone and VX680-a well-known Aurora kinase inhibitor-and we found that these profiles exhibited considerable similarity in cell morphology, growth, and death. Additionally, Derrone significantly inhibited the formation and growth of MCF7 tumor spheroids. |
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