| In vitro: |
| Microbiology China,2010, 37(10):1475-80. | | The Endophytic Fungus Strain FTJZZJ09 Isolated from the Bulbs of Fritillaria thunbergii and Its Antibacterial Metabolites[Reference: WebLink] |
METHODS AND RESULTS:
In the present study, an endophytic fungus isolate FTJZZJ09, which isolated from the fresh bulbs of Fritillaria thunbergii Miq., was identified as Penicillium chrysogenum based on its morphological characters and internal transcribed spacer (ITS) sequence. After being cultured in the modified CzapeK-DoX medium (3 g/L maltose, 3 g/L peptone A, 0.1 g/L K2HPO4, 0.05 g/L KCl, 0.3 g/L NaNO3, 0.05 g/L MgSO4·7H2O, 0.001 g/L FeSO4·7H2O, pH 6.5), it can secrete antibacterial metabolites under the condition of 28°C in a rotary shaker at 160 r/min for 7 days. Three antibacterial compounds were isolated from the ethyl acetate extract of the fermentation broth by silica gel, they were elucidated as Cyclo(Pro-Gly) , cyclo (Pro-Val) and 2-acetyl-4 (3H) quinazolinone.
CONCLUSIONS:
All the three compounds could inhibit the growth of Bacillus subtilis with the minimal inhibitory concentration (MIC) value of 0.8, 0.8, and 0.4 g/L respectively, while they showed no apparent effects against the growth of Gram-negative bacteria. | | Chinese Journal of Antibiotics, 2006, 31(1):36-38,62. | | The antitumor active component from marine derived actinomycete S1001[Reference: WebLink] |
METHODS AND RESULTS:
To explore the antitumor active component in fermentation of a marine derived actinomycete S1001,8 compounds were isolated and purified by using solvent extraction,silica gel column and preparative HPLC.The separation procedure was traced by a bioassay using tsFT210 cell lines.By means of physico-chemical properties and spectral analyses,the structures of compounds were determined as: 4′,5,7-trihydroxyisoflavone(),4-(2,4-dihydroxybenzamido) benzoic acid (2),cyclo-(Gly-Ala)(3),Cyclo(Pro-Gly) (4),(cyclo)-(Leu-Tyr)(5),cyclo-(Val-Leu)(6),cyclo-(Val-Ile)(7),cyclo-(Phe-Gly)(8),respectively.
CONCLUSIONS:
The antitumor activities of these compounds were assayed by the SRB method against K562 cell line.Compounds 4, 5 and 7 showed cytotoxicity at the concentration of 10 μmol/L. |
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