黄芪皂苷II

Astragaloside II

	产品编号	CFN99173
	CAS编号	84676-89-1
	分子式 = 分子量	C₄₃H₇₀O₁₅ = 827.02
	产品纯度	>=98%
	物理属性	Powder
	化合物类型	Triterpenoids
	植物来源	The roots of Astragalus membranaceus (Fisch.) Bunge
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产品名称	产品编号	CAS编号	包装	QQ客服
黄芪皂苷II	CFN99173	84676-89-1	10mg	QQ客服：2159513211
黄芪皂苷II	CFN99173	84676-89-1	20mg	QQ客服：2159513211
黄芪皂苷II	CFN99173	84676-89-1	50mg	QQ客服：2159513211
黄芪皂苷II	CFN99173	84676-89-1	100mg	QQ客服：2159513211

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Description:

Astragaloside II is a potent autophagy inhibitor and multidrug resistance (MDR) reversal agent, which restores chemosensitivity of anticancer agent cisplatin and enhances tumor cell death. It has immunomodulating activity, can trigger T cell activation through regulation of CD45 protein tyrosine phosphatase activity, it induces osteogenic activities of osteoblasts through the bone morphogenetic protein-2/MAPK and Smad1/5/8 pathways.

Targets:

gp120/CD4 | IL Receptor | p38MAPK | ERK | PI3K | Akt | mTOR

In vitro:

J Pharm Pharmacol. 2012 Dec;64(12):1741-50.

Reversal of P-glycoprotein-mediated multidrug resistance of human hepatic cancer cells by Astragaloside II.[Pubmed: 23146037]

Chemoresistance is the main obstacle encountered in cancer treatment and is frequently associated with multidrug resistance (MDR). Astragaloside is a saponin which is widely used in traditional Chinese medicine. It has been reported that Astragaloside has antitumour effects on hepatocellular carcinoma Bel-7402 cells in vitro and in vivo. The purpose of this study was to examine the effects of Astragaloside II on the reversal of MDR and its molecular mechanism in vitro.
METHODS AND RESULTS:
In this study, Bel-7402 and Bel-7402/FU cell lines were used as the experimental model. Drug sensitivity was determined using the 3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide assay, accumulation and efflux of Rh123 were analyzed by flow cytometer, the mRNA level of mdr1 was determined by RT-PCR and the protein levels of P-glycoprotein (P-gp) and mitogen-activated protein kinase were determined by Western blot. Astragaloside II (0.08 mg/ml) showed strong potency to increase 5-fluorouracil cytotoxicity toward 5-fluorouracil-resistant human hepatic cancer cells Bel-7402/FU. The mechanism of Astragaloside II on P-gp-mediated MDR demonstrated that Astragaloside II significantly increased the intracellular accumulation of rhodamine 123 via inhibition of P-gp transport function. Based on the analysis of P-gp and mdr1 gene expression using Western blot and RT-PCR, the results revealed that Astragaloside II could downregulate the expression of the P-gp and mdr1 gene. In addition, Astragaloside II suppressed phosphorylation of extracellular signal regulated kinase 1/2, p38 and c-Jun N-terminal kinase.
CONCLUSIONS:
The results suggested that Astragaloside II is a potent MDR reversal agent and may be a potential adjunctive agent for hepatic cancer chemotherapy.

In vivo:

Acta Pharmacol Sin. 2013 Apr;34(4):522-30.

Astragaloside II triggers T cell activation through regulation of CD45 protein tyrosine phosphatase activity.[Pubmed: 23524573]

To investigate the immunomodulating activity of astragalosides, the active compounds from a traditional tonic herb Astragalus membranaceus Bge, and to explore the molecular mechanisms underlying the actions, focusing on CD45 protein tyrosine phosphatase (CD45 PTPase), which plays a critical role in T lymphocyte activation.
METHODS AND RESULTS:
Primary splenocytes and T cells were prepared from mice. CD45 PTPase activity was assessed using a colorimetric assay. Cell proliferation was measured using a [(3)H]-thymidine incorporation assay. Cytokine proteins and mRNAs were examined with ELISA and RT-PCR, respectively. Activation markers, including CD25 and CD69, were analyzed using flow cytometry. Activation of LCK (Tyr505) was detected using Western blot analysis. Mice were injected with the immunosuppressant cyclophosphamide (CTX, 80 mg/kg), and administered astragaloside II (50 mg/kg). Astragaloside I, II, III, and IV concentration-dependently increased the CD45-mediated of pNPP/OMFP hydrolysis with the EC50 values ranged from 3.33 to 10.42 μg/mL. Astragaloside II (10 and 30 nmol/L) significantly enhanced the proliferation of primary splenocytes induced by ConA, alloantigen or anti-CD3. Astragaloside II (30 nmol/L) significantly increased IL-2 and IFN-γ secretion, upregulated the mRNA levels of IFN-γ and T-bet in primary splenocytes, and promoted CD25 and CD69 expression on primary CD4(+) T cells upon TCR stimulation. Furthermore, astragaloside II (100 nmol/L) promoted CD45-mediated dephosphorylation of LCK (Tyr505) in primary T cells, which could be blocked by a specific CD45 PTPase inhibitor. In CTX-induced immunosuppressed mice, oral administration of astragaloside II restored the proliferation of splenic T cells and the production of IFN-γ and IL-2. However, astragaloside II had no apparent effects on B cell proliferation.
CONCLUSIONS:
Astragaloside II enhances T cell activation by regulating the activity of CD45 PTPase, which may explain why Astragalus membranaceus Bge is used as a tonic herb in treating immunosuppressive diseases.

产品名称	产品编号	CAS编号	分子式 = 分子量	位单	联系QQ
黄芪皂苷III; Astragaloside III	CFN99174	84687-42-3	C₄₁H₆₈O₁₄ = 784.97	20mg	QQ客服：1413575084
黄肉楠碱; Actein	CFN99864	18642-44-9	C₃₇H₅₆O₁₁ = 676.9	5mg	QQ客服：1413575084
升麻素苷; Cimicifugoside	CFN90481	66176-93-0	C₃₇H₅₄O₁₁ = 674.81	5mg	QQ客服：1457312923
26-脱氧升麻苷; 26-Deoxycimicifugoside	CFN90788	214146-75-5	C₃₇H₅₄O₁₀ = 658.8	5mg	QQ客服：1413575084
23-表-26-脱氧黄肉楠碱; 23-epi-26-Deoxyactein	CFN91896	264624-38-6	C₃₇H₅₆O₁₀ = 660.83	5mg	QQ客服：1413575084
土荆皮内酯B; Pseudolarolide B	CFN91878	151368-43-3	C₃₀H₄₂O₄ = 466.65	5mg	QQ客服：1457312923
升麻内酯A; Cimilactone A	CFN92971	468733-06-4	C₃₃H₅₀O₉ = 590.74	5mg	QQ客服：215959384
21,24-环氧基环安坦-3,25-二醇; 21,24-Epoxycycloartane-3,25-diol	CFN97836	125305-73-9	C₃₀H₅₀O₃ = 458.73	5mg	QQ客服：215959384
环黄芪醇; Cycloastragenol	CFN99538	84605-18-5	C₃₀H₅₀O₅ = 490.71	20mg	QQ客服：3257982914
黄芪甲苷IV; 黄芪皂苷Ⅳ; 黄芪甲苷; 黄芪甲甙; Astragaloside IV	CFN99171	84687-43-4	C₄₁H₆₈O₁₄ = 784.98	20mg	QQ客服：2159513211

	1 mg	5 mg	10 mg	20 mg	25 mg
1 mM	1.2092 mL	6.0458 mL	12.0916 mL	24.1832 mL	30.229 mL
5 mM	0.2418 mL	1.2092 mL	2.4183 mL	4.8366 mL	6.0458 mL
10 mM	0.1209 mL	0.6046 mL	1.2092 mL	2.4183 mL	3.0229 mL
50 mM	0.0242 mL	0.1209 mL	0.2418 mL	0.4837 mL	0.6046 mL
100 mM	0.0121 mL	0.0605 mL	0.1209 mL	0.2418 mL	0.3023 mL

黄芪皂苷II

Astragaloside II

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