| In vitro: |
| Journal of Biosafety, 2011, 20 (3) :207-12. | | Isolation and identification of a potential allelochemical from the invasive plant Eupatorium adenophorum[Reference: WebLink] |
METHODS AND RESULTS:
The chemical structures of the purified compounds were identified by spectroscopy, including MS and NMR techniques. The allelopathic potential of sub-fractions of EtOAc extract and isolated pure compounds as well as two literature reported terpenoid allelochemicals, 8-Hydroxy-4-cadinen-3-one (2) and 4,7(11)-cadinadiene-3,8-dione (3), and signaling compound JA-Ile, were all evaluated by detecting their inhibitory activity against seeds germination and seedling root growth using Arabidopsis thaliana as test plant.
A bioactive compound was isolated from one polar sub-fraction of EtOAc extract of E.adenophorum and its chemical structure was identified as 2-coumaric acid glucoside (1). Bioassay indicated that compound 1 showed significant inhibitory activity against A.thaliana seedling root growth at concentration as low as 0.1 mmol·L-1, which is more potent than the other two identified compounds. A closely similar inhibition pattern of 1 to that of the signaling compound JA-Ile were also observed.
CONCLUSIONS:
In addition to less polar terpenoids, potentially existing large polar allelochemicals in E.adenophorum might significantly contribute the total allelopathic effects. The discovery of compound 1 and other potential polar allelochemicals from E.adenophorum may significantly promote the elucidation of the potential allelopathic mechanism possessed by this well-known invasive plant. |
|
Cell. 2018 Jan 11;172(1-2):249-261.e12. doi: 10.1016/j.cell.2017.12.019.IF=36.216(2019)
Cell Metab. 2020 Mar 3;31(3):534-548.e5. doi: 10.1016/j.cmet.2020.01.002.IF=22.415(2019)
Mol Cell. 2017 Nov 16;68(4):673-685.e6. doi: 10.1016/j.molcel.2017.10.022.IF=14.548(2019)
ACS Nano. 2018 Apr 24;12(4): 3385-3396. doi: 10.1021/acsnano.7b08969.IF=13.903(2019)
Nature Plants. 2016 Dec 22;3: 16206. doi: 10.1038/nplants.2016.205.IF=13.297(2019)
Sci Adv. 2018 Oct 24;4(10): eaat6994. doi: 10.1126/sciadv.aat6994.IF=12.804(2019)
