| In vitro: |
| Int J Mol Sci. 2012;13(2):1762-77. | | 10-Shogaol, an antioxidant from Zingiber officinale for skin cell proliferation and migration enhancer.[Pubmed: 22408422] |
METHODS AND RESULTS:
In this work, one of Zingiber officinale components, 10-Shogaol, was tested with 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical scavenging, metal chelating ability, and reducing power to show antioxidant activity. 10-Shogaol promoted human normal epidermal keratinocytes and dermal fibroblasts cell growths. 10-Shogaol enhanced growth factor production in transforming growth factor-β (TGF-β), platelet derived growth factor-αβ (PDGF-αβ) and vascular endothelial growth factors (VEGF) of both cells. In the in vitro wound healing assay for 12 or 24 h, with 10-Shogaol, the fibroblasts and keratinocytes migrated more rapidly than the vehicle control group.
CONCLUSIONS:
Thus, this study substantiates the target compound, 10-Shogaol, as an antioxidant for human skin cell growth and a migration enhancer with potential to be a novel wound repair agent. | | J Agric Food Chem. 2014 May 21;62(20):4632-42. | | Cysteine-conjugated metabolites of ginger components, shogaols, induce apoptosis through oxidative stress-mediated p53 pathway in human colon cancer cells.[Pubmed: 24786146] | Shogaols, the major constituents of thermally processed ginger, have been proven to be highly effective anticancer agents.
METHODS AND RESULTS:
Our group has identified cysteine-conjugated shogaols (M2, M2', and M2″) as the major metabolites of [6]-, [8]-, and 10-Shogaol in human and found that M2 is a carrier of its parent molecule [6]-shogaol in cancer cells and in mice, while being less toxic to normal colon fibroblast cells. The objectives of this study are to determine whether M2' and M2″ behave in a similar manner to M2, in both metabolism and efficacy as anticancer agents, and to further explore the biological pro-apoptotic mechanisms of the cysteine-conjugated shogaols against human colon cancer cells HCT-116 and HT-29.
CONCLUSIONS:
Our results show that [8]- and 10-Shogaol have similar metabolic profiles to [6]-shogaol and exhibit similar toxicity toward human colon cancer cells. A brief screen of the markers attenuated by the proapoptotic activity of M2 revealed similar results for [8]- and 10-Shogaol and their respective cysteine-conjugated metabolites M2' and M2″. This study highlights the cysteine-conjugated metabolites of shogaols as novel dietary colon cancer preventive agents. |
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