| In vivo: |
| Planta Med., 2008, 74(9):983-4. | | Three anti-inflammatory stigmastane steroids from Alchornea floribunda leaves[Reference: WebLink] | The anti-inflammatory activities of these compounds were evaluated using in vitro and in vivo animal models.
METHODS AND RESULTS:
The compounds, 1, 2 and 3 at 5mg/ear inhibited xylene – induced ear edema in mice, with edema inhibitions of 59.9, 51.5 and 51.54% respectively. At 20mg/kg (ip), all the compounds significantly (p<0.05) inhibited acute inflammation induced by subplanta injection of undiluted egg albumen in rats' paw. Compound 1 exhibited a higher anti-inflammatory effect (% edema inhibition=50. 0) than prednisolone (% edema inhibition=48.0) at 3h. 1 and 3 at concentrations of 50 and 100μg/ml significantly (p<0.05) stabilized heat-induced haemolysis of human erythrocytes in vitro, but have no effect on hypotonicity-induced haemolysis. Spectral analysis elucidated the compounds as Stigmastane-3,6-dione (1), stigmastane-22-ene-3,6-dione (2) and 3-hydroxystigmastane-22-ene (3).
CONCLUSIONS:
These compounds may, in part, account for the anti-inflammatory effect of Alchornea floribunda leaves. |
|
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