| In vitro: |
| Pak J Pharm Sci. 2005 Apr;18(2):3-5. | | Anti-bacterial alkaloids of Saracococca saligna.[Pubmed: 16431389] |
METHODS AND RESULTS:
The crude ethanolic extract of Saracococca saligna was found to be cytotoxic and antibacterial but produced no platelet aggregation induced by ADP. Antibacterial activity of two known alkaloid, pachyaximine-A and saracodine was determined.
CONCLUSIONS:
Pachyaximine-A possessed significant antibacterial activity against Escherichia coli, Staphylococcus aureus, Corynebacterium diphtheriae and Corynebacterium pyrogenes. Saracodme showed moderate activity against Staphylococcus pyrogenes, Escherichia coli, Staphylococcus aureus, Corynebacterium diphtheriae, Shigella boydii and Klebsiella pneumoniae. | | J Nat Prod. 1998 Oct;61(10):1257-62. | | Activity-guided isolation of steroidal alkaloid antiestrogen-binding site inhibitors from Pachysandra procumbens.[Pubmed: 9784163 ] |
METHODS AND RESULTS:
Four novel steroidal alkaloids, (+)-(20S)-20-(dimethylamino)-3-(3'alpha-isopropyl)-lactam-5alpha-+ ++preg n-2-en-4-one (1), (+)-(20S)-20-(dimethylamino)-16alpha-hydroxy-3-(3'alpha-isopropyl) -la ctam-5alpha-pregn-2-en-4-one (2), (+)-(20S)-3-(benzoylamino)-20-(dimethylamino)-5alpha-pregn-2-en-++ +4beta -yl acetate (3), and (+)-(20S)-2alpha-hydroxy-20-(dimethylamino)-3beta-phthalimido-5 alpha- pregnan-4beta-yl acetate (4), as well as five known compounds, (-)-Pachyaximine A (5), (+)-spiropachysine (6), (+)-axillaridine A (7), (+)-epipachysamine D (8), and (+)-pachysamine B (9), were isolated from Pachysandra procumbens, using a bioassay-guided fractionation based on inhibition of 3H-tamoxifen binding at the antiestrogen binding site (AEBS).
CONCLUSIONS:
Compounds 1-7 and 9 demonstrated significant activity as AEBS-inhibitory agents, and compounds 3, 5 and 9 were found to potentiate significantly the antiestrogenic effect mediated by tamoxifen in cultured Ishikawa cells. The structure elucidation of compounds 1-4 was carried out by spectral data interpretation. |
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