| In vivo: |
| Chem.Biol. Interact., 1975, 10(3):185-97. | | Localization in the cell cycle of the antimitotic action of the pyrrolizidine alkaloid, lasiocarpine and of its metabolite, dehydroheliotridine[Reference: WebLink] |
METHODS AND RESULTS:
The antimitotic action of the pyrrolizidine alkaloid lasiocarpine on rat liver parenchyma was investigated using as the experimental model the wave of mitosis produced in liver by a single dose of thioacetamide. A single low dose of lasiocarpine administered two weeks before the thioacetamide, almost completely inhibited the mitotic wave without inhibiting to the same extent the preceding wave of DNA synthesis. By the use of selective inhibitors and radioisotope labelling, the location of the mitotic block was found to be either in the latter half of the DNA synthetic phase, S, or early in G2, the post-synthetic phase.
CONCLUSIONS:
The mitotic wave was similarly inhibited by pretreatment of the rats with a single injection of dehydroheliotridine, a pyrrolic metabolite of heliotridine-based pyrrolizidine alkaloids. | | J.Pathol. Bacteriol., 1959, 78(2):483–502. | | The chronic pathological effects on the liver of the rat of the pyrrolizidine alkaloids heliotrine, lasiocarpine, and their N -oxides[Pubmed: 13805866] |
METHODS AND RESULTS:
Pure crystalline alkaloids and N-oxides used were heliotrine, lasiocarpine, heliotrine N-oxide, lasiocarpine N-oxide, heliotridine and heliotric acid. Young male rats, bodyweight 120 to 220 g., were given intraperitoneal injections of the alkaloids; in preliminary experiments the dose was 0.1 LD50 twice or three times a week, which produced liver damage and caused death generally in about 6 mon... |
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