Isoline

	产品编号	CFN00351
	CAS编号	30000-36-3
	分子式 = 分子量	C₂₀H₂₉NO₇ = 395.45
	产品纯度	>=98%
	物理属性	Cryst.
	化合物类型	Alkaloids
	植物来源	The herbs of Senecio othonniformis Fourcade.
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产品名称	产品编号	CAS编号	包装	QQ客服
Isoline	CFN00351	30000-36-3	1mg	QQ客服：3257982914
Isoline	CFN00351	30000-36-3	5mg	QQ客服：3257982914
Isoline	CFN00351	30000-36-3	10mg	QQ客服：3257982914
Isoline	CFN00351	30000-36-3	20mg	QQ客服：3257982914

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Description:

Isoline can induce different oxidative injury in various important mouse organs, and of which liver is the most sensitive organ. Isoline decreases the cellular GSH and the ratio of GSH and oxidizes glutathione in a time- and concentration-dependent manner in L-02 cells. l-Buthionine-(S, R)-sulfoximine (BSO) and mercaptosuccinic acid (MA), inhibitors of GCL and GPx, both augmented Isoline-induced cytotoxicity in cultured mice hepatocytes.

Targets:

ATPase | GSH | GPx | GCL

In vitro:

Toxicol Ind Health. 2013 Jul;29(6):567-75.

Involvement of intracellular glutathione in regulating isoline-induced cytotoxicity in human normal liver L-02 cells.[Pubmed: 22474030]

Pyrrolizidine alkaloid isoline is isolated from the traditional Chinese medicine Ligularia duciformis. Our previous reports have already demonstrated isoline-induced liver injury in mice.
METHODS AND RESULTS:
The present study is designed to observe the involvement of intracellular reduced glutathione (GSH) in isoline-induced cytotoxicity in human normal liver L-02 cells. The results showed that isoline decreased the cellular GSH and the ratio of GSH and oxidized glutathione in a time- and concentration-dependent manner in L-02 cells. l-Buthionine-S-R-sulfoximine (BSO) is reported to inhibit cellular GSH biosynthesis, and further results showed that isoline decreased the cell viability in L-02 cells after pretreated with 25 μM BSO for 24 h. Furthermore, adducts of isoline and GSH were identified in L-02 cells using liquid chromatography/electrospray ionization tandem mass spectrometry (ion trap) for the first time.
CONCLUSIONS:
In conclusion, our study provides the strongest evidence to support the important roles of GSH in regulating isoline-induced cytotoxicity in human normal liver L-02 cells.

In vivo:

Environ Toxicol Pharmacol. 2012 Sep;34(2):608-17.

Proteomic characterization of the possible molecular targets of pyrrolizidine alkaloid isoline-induced hepatotoxicity.[Pubmed: 22885678]

Pyrrolizidine alkaloids (PAs) are distributed in plants worldwide including medicinal herbs or teas. In the present study, we investigated the effects of isoline, which is a retronecine-type PA isolated from traditional Chinese medicinal herb Ligularia duciformis, on mouse liver proteins by using proteomic approaches.
METHODS AND RESULTS:
Firstly, our results showed that 110mg/kg isoline increased alanine aminotransferase (ALT) and aspartate aminotransferase (AST) activities in serum, and hepatic tissue pathological observation further confirmed isoline-induced liver injury. Proteomic analysis showed that the liver samples from mice of isoline group demonstrated about 13 differentially expressed proteins compared with normal group, and those proteins may be involved in isoline-induced liver injury in mice. Next, all these 13 protein spots were identified by MALDI-TOF-TOF MS or LTQ MS; and among them 9 differentially expressed proteins are involved in the process of oxidative stress or cellular energy metabolism. Further lipid peroxidation analysis and ATPase assay confirmed the existing of oxidative injury induced by isoline and consequent disruption of energy metabolism. Furthermore, an in silico drug target searching program INVDOCK identified 2 potential protein targets of isoline, and the results are in support of proteomic analysis.
CONCLUSIONS:
In summary, the possible signaling molecules related with isoline-induced liver injury were demonstrated in this study.

Arch Toxicol. 2011 Oct;85(10):1267-79.

The difference of glutathione antioxidant system in newly weaned and young mice liver and its involvement in isoline-induced hepatotoxicity.[Pubmed: 21327617]

Cellular glutathione antioxidant system plays important roles in counteracting hepatotoxins-induced oxidative stress injury. The present study was designed to observe the differences of this system in newly weaned and young mice liver and its involvement in the susceptibility to isoline-induced liver injury.
METHODS AND RESULTS:
Our results showed that liver reduced glutathione (GSH) amounts were higher in newly weaned mice than young mice. Glutamate-cysteine ligase (GCL) activity was higher in newly weaned mice due to the higher expression of catalytic subunit of GCL (GCLC) protein and mRNA. However, the activities of glutathione reductase (GR), glutathione peroxidase (GPx), and glutathione-S-transferase (GST) were higher in young mice liver, which might be due to the higher expression of GR, GPx-1, and GST-Pi proteins. Next, the results of AST analysis and histopathological evaluation showed that newly weaned mice demonstrated more severe liver injury induced by isoline. Furthermore, liver GSH amounts and the activities of GR, GPx, and GST were all lower in newly weaned mice than young mice after treated with isoline. Depletion of cellular GSH by D,L -buthionine-(S, R)-sulfoximine (BSO) aggravated isoline-induced cytotoxicity, while N-acetyl-l cysteine (NAC) ameliorated such cytotoxicity. Furthermore, the inhibitors of GR, GPx, and GST all aggravated isoline-induced cytotoxicity.
CONCLUSIONS:
In conclusion, our results demonstrated the differences of glutathione antioxidant system between newly weaned and young mice liver. Meanwhile, our results also revealed age-dependent liver injury induced by isoline for the first time, which might be due to the different responses of glutathione antioxidant system to isoline between newly weaned and young mice.

	1 mg	5 mg	10 mg	20 mg	25 mg
1 mM	2.5288 mL	12.6438 mL	25.2876 mL	50.5753 mL	63.2191 mL
5 mM	0.5058 mL	2.5288 mL	5.0575 mL	10.1151 mL	12.6438 mL
10 mM	0.2529 mL	1.2644 mL	2.5288 mL	5.0575 mL	6.3219 mL
50 mM	0.0506 mL	0.2529 mL	0.5058 mL	1.0115 mL	1.2644 mL
100 mM	0.0253 mL	0.1264 mL	0.2529 mL	0.5058 mL	0.6322 mL

产品名称	产品编号	CAS编号	分子式 = 分子量	位单	联系QQ
Emiline; Emiline	CFN00368	36506-99-7	C₁₉H₂₇NO₆ = 365.42	5mg	QQ客服：215959384
夹可灵; Jacoline	CFN00376	480-76-2	C₁₈H₂₇NO₇ = 369.41	5mg	QQ客服：2056216494
夹可灵 N-氧化物; Jacoline N-oxide	CFN00462	1148039-73-9	C₁₈H₂₇NO₈ = 385.4	5mg	QQ客服：2159513211
夹可宁; Jaconine	CFN00377	480-75-1	C₁₈H₂₆ClNO₆ = 387.86	5mg	QQ客服：215959384
Onetine; Onetine	CFN00390	41451-67-6	C₁₉H₂₉NO₈ = 399.44	5mg	QQ客服：2056216494
Floricaline; Floricaline	CFN00392	16958-32-0	C₂₃H₃₃NO₁₀ = 483.51	5mg	QQ客服：2056216494
Anonamine; Anonamine	CFN00427	111566-66-6	C₁₉H₂₇NO₇ = 381.42	5mg	QQ客服：2159513211
Merenskine; Merenskine	CFN00465	96657-94-2	C₁₈H₂₆ClNO₆ = 387.9	5mg	QQ客服：3257982914
Merenskine N-oxide; Merenskine N-oxide	CFN00466	96657-95-3	C₁₈H₂₆ClNO₇ = 403.9	5mg	QQ客服：1457312923
Merepoxine; Merepoxine	CFN00467	115777-94-1	C₁₈H₂₅NO₆ = 351.4	5mg	QQ客服：1457312923

Isoline

Isoline

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