| In vitro: |
| Int J Radiat Biol. 2014 Jul;90(7):575-9. | | Effect of photodynamic therapy with hypocrellin B on apoptosis, adhesion, and migration of cancer cells.[Pubmed: 24661233] | In the present study, we investigated effects of photodynamic therapy with Hypocrellin B on apoptosis, adhesion, and migration of cancer cells in vitro.
METHODS AND RESULTS:
Human ovarian cancer HO-8910 cell as a cancer model cell was incubated with Hypocrellin B at a concentration of 2.5 μM for 5 h and irradiated by light from a light-emitting diodes (LED) source. Cell apoptosis was analyzed by flow cytometry with annexin V/propidium iodide (PI) staining and nuclear staining 6 h after Hypocrellin B photoirradiation. Cell adhesion was assessed using the 3-(4, 5-dimthylthiazol-2-yl)-2, 5 diphenyl-tetrazolium bromide (MTT) assay 4 h after photodynamic treatment. Cell migration was measured 48 h after photodynamic treatment. Flow cytometry with annexin V/PI staining showed that early apoptotic and late apoptotic (necrotic) rates following photodynamic therapy with Hypocrellin B markedly increased to 16.40% and 24.67%, respectively. Nuclear staining found nuclear condensation and typical apoptotic body in the treated cells. The number of cell migration was significantly decreased to 183 ± 28 after photodynamic therapy with Hypocrellin B (p < 0.01). Light irradiation alone and Hypocrellin B alone had no significant effect on cell migration. The cell adhesion inhibitory rate due to photodynamic action of Hypocrellin B was 53.2 ± 1.8%, significantly higher than 2.7 ± 2.1% of light treatment alone and 1.0 ± 0.4% of Hypocrellin B treatment alone (p < 0.01).
CONCLUSIONS:
The findings demonstrated that photodynamic therapy with Hypocrellin B remarkably induced apoptosis and inhibited adhesion and migration of cancer cells in vitro. | | Ultrasonics. 2012 Apr;52(4):543-6. | | Hypocrellin B-mediated sonodynamic action induces apoptosis of hepatocellular carcinoma cells.[Pubmed: 22172458] | The present study aims to investigate apoptosis of hepatocellular carcinoma cells induced by Hypocrellin B-mediated sonodynamic action.
METHODS AND RESULTS:
The Hypocrellin B concentration was kept constant at 2.5 μM and cells from the hepatocellular carcinoma HepG2 cell line were exposed to ultrasound with an intensity of 0.46 W/cm(2) for 8s. Cell cytotoxicity was quantified using an MTT assay 24 h after sonodynamic therapy (SDT) of Hypocrellin B. Apoptosis was investigated using a flow cytometry with Annexin V-FITC and propidium iodine staining. Intracellular reactive oxygen species (ROS) levels were detected using a flow cytometry with 2,7-dichlorodihydrofluorecein diacetate (DCFH-DA) staining. The cytotoxicity of Hypocrellin B-mediated sonodynamic action on HepG2 cells was significantly higher than those of other treatments including ultrasound alone, Hypocrellin B alone and sham treatment. Flow cytometry showed that Hypocrellin B-induced sonodynamic action markedly enhanced the apoptotic rate of HepG2 cells. Increased ROS was observed in HepG2 cells after being treated with Hypocrellin B-mediated sonodynamic action.
CONCLUSIONS:
Our data demonstrated that Hypocrellin B-mediated sonodynamic action remarkably induced apoptosis of HepG2 cells, suggesting that apoptosis is an important mechanism of cell death induced by Hypocrellin B-mediated SDT. |
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