| In vitro: |
| Journal of Shenyang Pharmaceutical University, 2007, 24(8):474-8. | | Cyclic dipeptide constituents from the mangrove fungus Penicillium oxalicum[Reference: WebLink] | To study the metabolites of mangrove fungus Penicillium oxalicum from the south China sea and search for new anti-tumor compounds.
METHODS AND RESULTS:
Compounds were isolated by silica gel,ODS column chromatography,Sephadex LH-20 column chromatography and reversed-phase HPLC purification.Structural elucidation was achieved by physico-chemical constants and spectroscopic analysis.MTT assay was used to evaluate the bioactivities in vitro.Six cyclic dipeptides were isolated from the acetone extracts of mycelium of fungus and elucidated as cyclo-(Phe-Ile)(1),cyclo(Phe-Val)(3),cyclo-(Ile-Leu)(3),cyclo-(Val-Val)(4),cyclo-(Pro-Val)(5),cyclo-(Pro-Gly)(6).Coumpouds 2,3 and 5 could evidently inhibit the growth of cancer cell lines HepG Ⅱ and LNCaP at the concentration of 50 μg·mL-1.
CONCLUSIONS:
All of Compounds are obtained from the marine fungus for the fist time.Compounds 2,3 and 5 show cytotoxic activity in vitro. | | Journal of Shenyang Pharmaceutical University, 2015 (2):107 -10. | | Cyclic dipeptides as new cell cycle inhibitors produced by Streptomyces flavoretus 18522[Reference: WebLink] | To find the cell cycle inhibitors from the metabolites of Streptomyces flavoretus 18522.
METHODS AND RESULTS:
Activity-guided isolation was performed on the chloroform extract of the fermentation broth and mycelia of Streptomyces flavoretus 18522 by using ts FT210 cells.Compounds were isolated through various chromatoghraphic methods and elucidated by spectroscopic means.The flow cytometry was used to evaluate the cell cycle inhibiting activity of the fractions and compounds. Six cyclic dipeptides were obtained and identified as cyclo(Ala-Leu)(1),cyclo(Ala-Ile)(2),cyclo(Ala-Val)(3),cyclo(Phe-Leu)(4),cyclo(Ala-Pro)(5) and Cyclo(Phe-Val)(6).
CONCLUSIONS:
Compounds 1-6 are reported as new cell cycle inhibitors for the first time. |
|
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