| In vitro: |
| Asian J. Pharm. Clin. Res., 2015, 8(5):221-6. | | Chemical composition and antimicrobial activity of diterpene and essential oils of hedychium roxburghii blume rhizome[Reference: WebLink] | The objective of the present study was to isolate and determine diterpene compound and essential oils from Hedychium roxburghii Blume rhizome and investigated those antimicrobial activities.
METHODS AND RESULTS:
The essential oils were obtained by steam distillation method, the residual was then extracted by reflux with ethanol. The content of essential oils was analyzed by gas chromatography-mass spectrometry (GC/MS) method. Ethanolic residual-distillation extract was concentrated then used to isolate compound 1 by vacuum liquid chromatography and centrifugal chromatography. It was characterized by infrared spectrophotometry, 1H-nuclear magnetic resonance (NMR), 13C-NMR, heteronuclear single quantum coherence-NMR, heteronuclear multiple bond correlation-NMR and carbon coupling 13C-NMR. The antimicrobial activity of essential oils, ethanolic residual-distillation extract and compound 1 were carried out by microdilution method. The oils exhibited antimicrobial activity against Bacillus subtilis American Type Culture Collection (ATCC) 6633 (minimum inhibitory concentration [MIC] 1750 μg/ml), Staphylococcus aureus ATCC 6538 (MIC 1750 μg/ml), Escherichia coli ATCC 8939 (MIC 3500 μg/ml), Pseudomonas aeruginosa ATCC 9027 (>3500 μg/ml) and Candida albicans ATCC 10231 (MIC 875 μg/ml). A phytochemical study of the rhizome essential oils of H. roxburghii Blume were performed by GC/MS and the result showed that fenchyl acetate (45.85%) was the main component of the oils. Compound 1 was identified as diterpene compound, coronarin E. Coronarin E have not exhibited MIC at 512 μg/ml, however, it showed inhibition profile against all of tested microbes.
CONCLUSIONS:
The essential oils and ethanolic residual-distillation extract of H. roxburghii Blume rhizome exhibited weak antimicrobial profile. Compound 1 was identified as diterpene compound, (coronarin E), it was exhibited weak antimicrobial activity, but showed inhibition profile against all of the tested microbes. |
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