| In vitro: |
| Letters in Applied Microbiology,2010,9(3):105-108. | | Evaluation of the antimicrobial activity of citral.[Reference: WebLink] |
METHODS AND RESULTS:
Citral showed appreciable antimicrobial activity against Gram-positive and Gram-negative bacteria as well as fungi. Media composition and inoculum size had no observable effect on activity but alkaline pH increased citral activity. The growth rates of Escherichia coli cultures were reduced at concentrations of citral ≥0·01% v/v while concentrations ≥0·03% v/v produced rapid reduction in viable cells followed by limited regrowth. In a non-growth medium, 0·08% and 0·1% v/v showed rapid bactericidal effects.
CONCLUSIONS:
Citral may therefore be of preservative use in addition to its other uses in the food, soap and cosmetic industries. | | Parasitology Research, 2009, 105(6):1489-96. | | In vitro activity of the essential oil of Cymbopogon citratus and its major component (citral) on Leishmania amazonensis.[Reference: WebLink] | Leishmaniasis causes considerable mortality throughout the world, affecting more than 12 million people. Cymbopogon citratus (DC) Stapf, Family Poaceae, is a widely used herb in tropical countries and is also known as a source of ethnomedicines.
METHODS AND RESULTS:
In this study, the inhibitory effect and the morphological and ultrastructural alterations on Leishmania amazonensis by the essential oil (EO) of C. citratus and its main constituent, citral, were evaluated. The results showed that the antiproliferative activity of EO on promastigotes and axenic amastigotes, and intracellular amastigote forms of L. amazonensis was significantly better than citral, and indicated a dose-dependent effect. Neither compound showed a cytotoxic effect on macrophage strain J774G8. The promastigote forms of L. amazonensis underwent remarkable morphological and ultrastructural alterations compared with untreated cultures. These alterations were visible by light, scanning, and transmission electron microscopy of promastigotes treated with EO and citral at concentrations corresponding to the IC50 (1.7 and 8.0 µg/ml) and IC90 (3.2 and 25 µg/ml), respectively, after 72 h of incubation.
CONCLUSIONS:
This study revealed that citral-rich essential oil from C. citratus has promising antileishmanial properties, and is a good candidate for further research to develop a new anti-protozoan drug. | | Fitoterapia, 2009, 80(5):290-296. | | Effect of citral, eugenol, nerolidol and α-terpineol on the ultrastructural changes of Trichophyton mentagrophytes.[Reference: WebLink] | The antifungal effects of citral, eugenol, nerolidol and α-terpineol on Trichophyton mentagrophytes were investigated.
METHODS AND RESULTS:
Citral over 0.1 mg/ml strongly inhibited the hyphal growth of T. mentagrophytes, and the antifungal activity of α-terpineol was less effective. The morphological changes of the fungus exposed to the terpenes were observed by electron microscopy. The hyphae were distorted and collapsed at 0.2, 0.4 and 1 mg/ml of eugenol, nerolidol and α-terpineol respectively, and cell membrane and organelles were irreversibly damaged at 0.2 mg/ml citral.
CONCLUSIONS:
These suggested that four terpenes possess antifungal activity against T. mentagrophytes, and the activity might lead to irreversible cellular disruption. |
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