| Zhong Yao Cai. 2014 Apr;37(4):632-5. |
| Regulative effect of bakuchiol on ESF-1 cells anti-aging gene[Pubmed: 25345139] |
To explore the mechanism of bakuchiol on anti-aging gene mRNA expression level of human skin fibroblasts (ESF-1).
METHODS AND RESULTS:
The potential of cell proliferation which was divided into blank group,positive control estradiol group, and bakuchiol high, medium and low dose groups was detected by MTT. The expression levels of Col I, Col III, TIMP-1, TIMP-2 and MMP-1 mRNA were detected with RT-PCR.
ESF-1 vitality and the expression levels of Col I, Col III, TIMP-1 and TIMP-2 mRNA were significantly increased by bakuchiol and E2. However, the expression of MMP-1 mRNA was reduced by bakuchiol and E2.
CONCLUSIONS:
The bakuchiol can enhance ESF-1 cell activity, promote collagen and matrix metalloproteinase inhibitors mRNA expression level and inhibit mRNA expression of matrix metalloproteinases in order to postpone skin aging. |
| Phytomedicine. 2014 Jun 15;21(7):942-5. |
| Anti-dermatophytic activity of bakuchiol: in vitro mechanistic studies and in vivo tinea pedis-inhibiting activity in a guinea pig model.[Pubmed: 24703327] |
Bakuchiol was an active antifungal compound isolated from Psoraleae Fructus by means of bioassay-guided fractionation in our previous study.
METHODS AND RESULTS:
The present work aimed to investigate the underlying mechanisms and the therapeutic effect of bakuchiol in Trichophyton mentagrophytes-induced tinea pedis. After exposure to bakuchiol at 0.25-fold, 0.5-fold and 1-fold of minimum inhibitory concentration (MIC) (3.91 μg/ml) for 24h, the fungal conidia of T. mentagrophytes demonstrated a significant dose-dependent increase in membrane permeability. Moreover, bakuchiol at 1-fold MIC elicited a 187% elevation in reactive oxygen species (ROS) level in fungal cells after a 3-h incubation. However, bakuchiol did not induce DNA fragmentation. In a guinea pig model of tinea pedis, bakuchiol at 1%, 5% or 10% (w/w) concentration in aqueous cream could significantly reduce the fungal burden of infected feet (p<0.01-0.05).
CONCLUSIONS:
In conclusion, this is the first report to demonstrate that bakuchiol is effective in relieving tinea pedis and in inhibiting the growth of the dermatophyte T. mentagrophytes by increasing fungal membrane permeability and ROS generation, but not via induction of DNA fragmentation. |
| Eur J Med Chem. 2012 Mar;49:55-67. |
| Bakuchiol derivatives as novel and potent cytotoxic agents: a report.[Pubmed: 22245048] |
METHODS AND RESULTS:
A library of 28 compounds comprising of acyl, amino, halo, nitro, styryl and cyclized derivatives of bakuchiol have been evaluated against a panel of eight human cancer cell lines. Bioevaluation studies have resulted in the identification of potent cytotoxic molecules exhibiting concentration dependent growth inhibition against leukemia cancer cells with best results observed for compounds 17 and 22 exhibiting IC(50) 1.8 and 2.0 μM respectively.
CONCLUSIONS:
As evident from various biological end-points, inhibition of cell proliferation by inducing G2/M cell cycle arrest, mitochondrial membrane disruption followed by DNA fragmentation and apoptosis is demonstrated. |
| Yao Xue Xue Bao. 2010 Apr;45(4):467-70. |
| Vitro antitumor activity and synthesis of the key intermediate of bakuchiol.[Pubmed: 21355211] |
METHODS AND RESULTS:
The in vitro antitumor activity of bakuchiol was exploited, compared with tamoxifen. The result of biological activities showed that bakuchiol could inhibit human breast cancer and the IC50 values were 2.89 x 10(-5) mol L(-1) and 8.29 x 10(-3) mol L(-1) against the cells line T-47D and MDA-MB-231 respectively. On the other hand, the key intermediate to synthesize bakuchiol was obtained by the method of Ireland-Claisen rearrangement.
CONCLUSIONS:
Comparing with traditional Claisen rearrangement, the reaction conditions are milder and the reaction reagents are safer. |
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Sci Adv. 2018 Oct 24;4(10): eaat6994. doi: 10.1126/sciadv.aat6994.IF=12.804(2019)
