| In vitro: |
| Science in China(Series B:Chemistry),2009, 52(3):332-337. | | Screening and docking studies of natural phenolic inhibitors of carbonic anhydrase II.[Reference: WebLink] | Carbonic anhydrase II (CAII) is an important enzyme complex with Zn2+,which is involved in many physiological and pathological processes, such as calcification, glaucoma and tumorigenicity.
METHODS AND RESULTS:
In order to search for novel inhibitors of CAII, inhibition assay of carbonic anhydrase II was performed, by which seven natural phenolic compounds, including four phenolics (grifolin, 4-O-Methylgrifolic acid, grifolic acid, and isovanillic acid) and three flavones (eriodictyol, quercetin and puerin A), showed in-hibitory activities against CAII with IC50s in the range of 6.37-71.73 umol/L. Grifolic acid is the most active one with IC50 of 6.37 umol/L.
CONCLUSIONS:
These seven phenolic compounds were proved to be novel natural carbonic anhydrase II inhibitors, which were obtained in flexible docking study with GOLD 3.0 soft-ware. Results indicated that the aliphatic chain and polar groups of hydroxyl and carboxyl are impor-tant to their inhibitory activities, providing a new insight into study on CA II potent inhibitors. |
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