| Description: |
4-Hydroxy-3,5-dimethoxybenzaldehyde is an organic compound that occurs naturally in trace amounts, it possesses powerful antioxidant activity, in minimizing DNA damage, and activating survival signal Akt pathway, and it may be of value in the development of radioprotective compounds. It is used in pharmaceuticals, food, cosmetics, textiles, pulp and paper industries, and even in biological control applications.
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| In vitro: |
| Eur J Pharm Sci. 2008 Jan;33(1):52-9. | | Radioprotection of 4-hydroxy-3,5-dimethoxybenzaldehyde (VND3207) in culture cells is associated with minimizing DNA damage and activating Akt.[Pubmed: 17981442] | Vanillin is a naturally occurring compound and food-flavoring agent with antioxidant and antimutagenic activities.
METHODS AND RESULTS:
In present study, we explored the radioprotective effect of a novel vanillin derivative VND3207 (4-Hydroxy-3,5-dimethoxybenzaldehyde). 4-Hydroxy-3,5-dimethoxybenzaldehyde has a much higher potential in scavenging hydroxyl radical and superoxide radical than vanillin as indicated in the ESR spin-trapping measurement, and it can effectively protect plasmid DNA against 10-50 Gy gamma-ray induced breaks in vitro at the concentrations as low as 10-20 microM. Using human lymphoblastoid AHH-1 cells and human fibroblastoid HFS cells, we demonstrated that 4-Hydroxy-3,5-dimethoxybenzaldehyde at 5-40 microM concentrations significantly attenuated the inhibition of proliferation and occurrence of apoptosis produced by 1-8 Gy gamma-irradiation. In the cultured cells, 4-Hydroxy-3,5-dimethoxybenzaldehyde significantly decreased the initial production and residual level of DNA double-strand breaks (DSBs) induced by 2 or 8 Gy irradiation. Treatment of 4-Hydroxy-3,5-dimethoxybenzaldehyde enhanced the level of DNA-PKcs protein, a critical component of DNA DSB repair pathway in the cells with or without gamma-irradiation. Consistently, the phosphorylation of Akt protein, a mediator of survival signal, as well as its substrate GSK3beta was concurrently increased by 4-Hydroxy-3,5-dimethoxybenzaldehyde.
CONCLUSIONS:
Our results suggest that 4-Hydroxy-3,5-dimethoxybenzaldehyde has radioprotection effect through its capabilities as a powerful antioxidant, in minimizing DNA damage, and activating survival signal Akt pathway, and it may be of value in the development of radioprotective compounds. |
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