| Description: |
4',7-Isoflavandiol, an estrogen metabolite, affects the ability of soy nuts to improve cardiovascular risk factors. 4',7-Isoflavandiol is a potential anticancer agent against HeLa, with possible mechanisms involved in ROS generation and mitochondrial membrane alteration; it may advance breast cancer potential via up-regulation of the eukaryotic initiation factor 4GI (eIF4GI). |
| Targets: |
c-Myc | ROS | MMP(e.g.TIMP) | Bcl-2/Bax | Caspase | Estrogen receptor |
| In vitro: |
| Anticancer Res. 2014 Sep;34(9):4985-92. | | Equol induces mitochondria-mediated apoptosis of human cervical cancer cells.[Pubmed: 25202081] | The present study aimed to investigate anticancer properties of 4',7-Isoflavandiol and demonstrate its underlying mechanisms of action in human cervical cancer HeLa cells.
METHODS AND RESULTS:
Inhibition of cell viability was examined by 3-(4,5-dimethylthiazoly-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay. Apoptosis was evaluated by observation of apoptotic cell morphology, and an increase of annexin-V(+) cells. Western blotting was used to examine apoptosis-related proteins. Flow cytometry was used to measure mitochondrial membrane potential (MMP) and reactive oxygen species (ROS). 4',7-Isoflavandiol treatment inhibited HeLa cell proliferation in dose- and time-dependent manner. 4',7-Isoflavandiol-induced apoptotic cell death was accompanied by the activation of caspases, and alteration of MMP and mitochondrial membrane proteins; 4',7-Isoflavandiol also rapidly triggered ROS production. Pre-treatment with N-acetylcysteine blocked loss of MMP, caused increase of Bcl-2-associated X protein (Bax)/B-cell lymphoma 2 (Bcl-2) ratio, caspase-8 activation, and apoptosis induced by equol.
CONCLUSIONS:
4',7-Isoflavandiol is a potential anticancer agent against HeLa, with possible mechanisms involved in ROS generation and mitochondrial membrane alteration. |
|
| In vivo: |
| Metabolism. 2015 Feb;64(2):236-43. | | Effect of soy nuts and equol status on blood pressure, lipids and inflammation in postmenopausal women stratified by metabolic syndrome status.[Pubmed: 25441251] | Soy has been associated with lower risk of cardiovascular disease in Asian countries which consume daily soy. Our study examined whether production of 4',7-Isoflavandiol , an estrogen metabolite, affected the ability of soy nuts to improve cardiovascular risk factors.
METHODS AND RESULTS:
Sixty postmenopausal women participated in a randomized, controlled, crossover trial of a Therapeutic Lifestyle Changes (TLC) diet alone and a TLC diet in which 0.5 cup of soy nuts (25 g of soy protein and 101 mg of aglycone isoflavones) replaced 25 g of nonsoy protein daily. Each diet was followed for 8 weeks at the end of which blood pressure (BP), lipid levels, adhesion molecules and inflammatory markers were measured. Women with MetS had significantly higher baseline body mass index (BMI), BP, triglycerides (TG), and soluble intercellular adhesion molecule (sICAM) than women without MetS. In women with MetS on the soy diet, significant reductions in diastolic BP (7.7%; P=0.02), TG (22.9%; P=0.02), C-reactive protein (CRP) (21.4%; P=0.01) and sICAM (7.3%; P=0.03) were noted among equol producers compared to levels on the TLC diet. No significant changes were noted in 4',7-Isoflavandiol nonproducers. Similarly, in women without MetS, only 4',7-Isoflavandiol producers had significant reductions in diastolic BP (3.3%, P=0.02) and CRP (30%, P=0.04). In contrast to women with MetS, TG and sICAM levels were not affected in women without MetS, a finding possibly related to lower baseline levels.
CONCLUSIONS:
Cardiovascular risk reduction with soy nuts is not uniform and may be greater among producers of 4',7-Isoflavandiol.. |
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