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  • 甲基原薯蓣皂苷

    Methyl protodioscin

    甲基原薯蓣皂苷
    产品编号 CFN99585
    CAS编号 54522-52-0
    分子式 = 分子量 C52H86O22 = 1063.23
    产品纯度 >=98%
    物理属性 White powder
    化合物类型 Steroids
    植物来源 The roots of Dioscorea opposita Thunb.
    ChemFaces的产品在影响因子大于5的优秀和顶级科学期刊中被引用
    提供自定义包装
    产品名称 产品编号 CAS编号 包装 QQ客服
    甲基原薯蓣皂苷 CFN99585 54522-52-0 10mg QQ客服:215959384
    甲基原薯蓣皂苷 CFN99585 54522-52-0 20mg QQ客服:215959384
    甲基原薯蓣皂苷 CFN99585 54522-52-0 50mg QQ客服:215959384
    甲基原薯蓣皂苷 CFN99585 54522-52-0 100mg QQ客服:215959384
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    ChemFaces的产品在许多优秀和顶级科学期刊中被引用

    Cell. 2018 Jan 11;172(1-2):249-261.e12.
    doi: 10.1016/j.cell.2017.12.019.
    IF=36.216(2019)

    PMID: 29328914

    Cell Metab. 2020 Mar 3;31(3):534-548.e5.
    doi: 10.1016/j.cmet.2020.01.002.
    IF=22.415(2019)

    PMID: 32004475

    Mol Cell. 2017 Nov 16;68(4):673-685.e6.
    doi: 10.1016/j.molcel.2017.10.022.
    IF=14.548(2019)

    PMID: 29149595

    ACS Nano. 2018 Apr 24;12(4): 3385-3396.
    doi: 10.1021/acsnano.7b08969.
    IF=13.903(2019)

    PMID: 29553709

    Nature Plants. 2016 Dec 22;3: 16206.
    doi: 10.1038/nplants.2016.205.
    IF=13.297(2019)

    PMID: 28005066

    Sci Adv. 2018 Oct 24;4(10): eaat6994.
    doi: 10.1126/sciadv.aat6994.
    IF=12.804(2019)

    PMID: 30417089
    我们的产品现已经出口到下面的研究机构与大学,并且还在增涨
  • Center for protein Engineering (CIP) (Belgium)
  • Fraunhofer-Institut für Molekularbiologie und Angewandte ?kologie IME (Germany)
  • University of Parma (Italy)
  • Instituto Politécnico de Bragan?a (Portugal)
  • Nanjing University of Chinese Medicine (China)
  • Universita' Degli Studi Di Cagliari (Italy)
  • Univerzita Karlova v Praze (Czech Republic)
  • VIT University (India)
  • Chang Gung University (Taiwan)
  • Shanghai Institute of Organic Chemistry (China)
  • Ain Shams University (Egypt)
  • Universite Libre de Bruxelles (Belgium)
  • University of Vienna (Austria)
  • Michigan State University (USA)
  • More...
  • 国外学术期刊发表的引用ChemFaces产品的部分文献
  • Phytochemistry Letters2015, 243-247
  • Sci Rep.2015, 5:13194
  • SBRAS2016, 12
  • J Pharm Anal.2016, 6(6):363-373
  • Semyung University2017, 149407
  • J Chromatogr A.2017, 1518:46-58
  • Int J Mol Sci.2017, 19(1)
  • Cell Physiol Biochem.2017, 43(4):1425-1435
  • J.Acta Agriculturae Scandinavica2017, 571-575
  • J Agric Food Chem.2017, 65(13):2670-2676
  • LWT-Food Science and Technology2017, 75:488-496
  • PLoS One.2018, 13(11):e0208055
  • Int J Mol Sci.2018, 19(9):E2528
  • J Cell Mol Med.2018, 22(9):4236-4242
  • J Sep Sci.2018, 41(11):2488-2497
  • Eur J Pharmacol.2018, 832:96-103
  • Front Pharmacol.2019, 10:1025
  • J of Physics Conference Series2019, 1349(1)
  • Int J Mol Sci.2019, 20(23):E6071
  • Molecules.2019, 24(12):E2286
  • J Food Biochem.2019, 43(9):e12970
  • Neurochem Int.2020, 133:104629
  • Molecules.2020, 25(3):734
  • ...
  • 生物活性
    Description: Methyl protodioscin has anti-thrombosis, antiosteoporotic, anti-myocardial infarction, and cytotoxic activities. Methyl protodioscin shows strong cytotoxicity against most cell lines from solid tumors with GI50 ≤10.0 microM, but moderate cytotoxicity is shown against leukemia cell lines with GI50 10-30 microM. It potentially increase HDL cholesterol while reducing LDL cholesterol and triglycerides, it also can treat diverse inflammatory pulmonary diseases.
    Targets: LDL | Caspase | Bcl-2/Bax
    In vitro:
    Pharmacogn Mag. 2014 Jul;10(39):318-24.
    Methyl protodioscin induces G2/M cell cycle arrest and apoptosis in A549 human lung cancer cells.[Pubmed: 25210320]
    Methyl protodioscin (MPD) is a furostanol bisglycoside with antitumor properties. It has been shown to reduce proliferation, cause cell cycle arrest. The present study elucidates the mechanism underlying MPD's apoptotic effects, using the A549 human lung cancer cell line.
    METHODS AND RESULTS:
    The human pulmonary adenocarcinoma cell line A549 was obtained from the Cell Bank of the Animal Experiment Center, North School Region, Sun Yat-Sen University. All of the cells were grown in RPMI 1640 supplemented with 10% fetal calf serum (Hyclone, Logan, UT, USA), penicillin (10,000 U/l), and streptomycin (100 mg/l) at 37°C in a 5% CO2 humidified atmosphere. The induction of apoptosis was observed in flow cytometry and fluorescent staining experiments. MPD showed growth inhibitory effects in A549 cells in a dose- and time-dependent manner. The significant G2/M cell cycle arrest and apoptotic effect were also seen in A549 cells treated with MPD. MPD-induced apoptosis was accompanied by a significant reduction of mitochondrial membrane potential, release of mitochondrial cytochrome c to cytosol, activation of caspase-3, downregulation of Bcl-2, p-Bad, and upregulation of Bax.
    CONCLUSIONS:
    Our results show that the induction of apoptosis by MPD involves multiple molecular pathways and strongly suggest that Bcl-2 family proteins signaling pathways. In addition, mitochondrial membrane potential, mitochondrial cytochrome c and caspase-3 were also closely associated with MPD-induced apoptotic process in human A549 cells.
    Cancer Invest. 2003 Jun;21(3):389-93.
    The cytotoxicity of methyl protodioscin against human cancer cell lines in vitro.[Pubmed: 12901285]
    Methyl protodioscin (NSC-698790) was a furostanol saponin isolated from the rhizome of Dioscorea collettii var. hypoglauca (Dioscoreaceae), a Chinese herbal remedy for the treatment of cervical carcinoma, carcinoma of the urinary bladder, and renal tumors for centuries.
    METHODS AND RESULTS:
    To systematically evaluate its potential anticancer activity, methyl protodioscin was tested cytotoxicity in vitro against human cancer cell lines by the NCI's (National Cancer Institute) anticancer drug screen. As a result, methyl protodioscin showed strong cytotoxicity against most cell lines from solid tumors with GI50 < or = 10.0 microM, especially selectively against one colon cancer line (HCT-15) and one breast cancer line (MDA-MB-435) with GI50 < 2.0 microM but moderate cytotoxicity was shown against leukemia cell lines with GI50 10-30 microM.
    CONCLUSIONS:
    The data are consistent with the fact that the rhizome of D. collettii var. hypoglauca has been employed for the treatment of solid tumors rather than leukemia in China for centuries. Based on an analysis using the COMPARE computer program with methyl protodioscin as a seed compound, no compounds in the NCI's anticancer drug screen database have cytotoxicity patterns similar to those of methyl protodioscin, indicating a potential novel mechanism of anticancer action.
    In vivo:
    Phytomedicine. 2015 May 15;22(5):568-72.
    Dioscin and methylprotodioscin isolated from the root of Asparagus cochinchinensis suppressed the gene expression and production of airway MUC5AC mucin induced by phorbol ester and growth factor.[Pubmed: 25981923 ]
    The root of Asparagus cochinchinensis (Lour.) Merr. has been utilized as mucoregulators and expectorants for controlling the airway inflammatory diseases in folk medicine. We investigated whether dioscin and methyl protodioscin isolated from the root of Asparagus cochinchinensis (Lour.) Merr. suppress the gene expression and production of airway MUC5AC mucin induced by phorbol ester and growth factor.
    METHODS AND RESULTS:
    Confluent NCI-H292 cells were pretreated with dioscin or methyl protodioscin for 30 min and then stimulated with EGF or PMA for 24 h. The MUC5AC mucin gene expression was measured by RT-PCR. Production of MUC5AC mucin protein was measured by ELISA. (1) Dioscin and methyl protodioscin suppressed the expression of MUC5AC mucin gene induced by EGF or PMA; (2) dioscin suppressed the production of MUC5AC mucin induced by either EGF at 10(-5) M (p < 0.05) and 10(-6) M (p < 0.05) or PMA at 10(-4) M (p < 0.05), 10(-5) M (p < 0.05) and 10(-6) M (p < 0.05); (3) methyl protodioscin also suppressed the production of MUC5AC mucin induced by either EGF at 10(-4) M (p < 0.05) or PMA at 10(-4) M (p < 0.05).
    CONCLUSIONS:
    These results suggest that dioscin and methyl protodioscin isolated from the root of Asparagus cochinchinensis suppress the gene expression and production of MUC5AC mucin, by directly acting on airway epithelial cells, and the results are consistent with the traditional use of Asparagus cochinchinensis as remedy for diverse inflammatory pulmonary diseases.
    制备储备液(仅供参考)
    1 mg 5 mg 10 mg 20 mg 25 mg
    1 mM 0.9405 mL 4.7027 mL 9.4053 mL 18.8106 mL 23.5133 mL
    5 mM 0.1881 mL 0.9405 mL 1.8811 mL 3.7621 mL 4.7027 mL
    10 mM 0.0941 mL 0.4703 mL 0.9405 mL 1.8811 mL 2.3513 mL
    50 mM 0.0188 mL 0.0941 mL 0.1881 mL 0.3762 mL 0.4703 mL
    100 mM 0.0094 mL 0.047 mL 0.0941 mL 0.1881 mL 0.2351 mL
    * Note: If you are in the process of experiment, it's need to make the dilution ratios of the samples. The dilution data of the sheet for your reference. Normally, it's can get a better solubility within lower of Concentrations.
    部分图片展示
    产品名称 产品编号 CAS编号 分子式 = 分子量 位单 联系QQ
    原皂苷Pb; Dichotomin CFN90703 53093-47-3 C57H94O26 = 1195.34 5mg QQ客服:3257982914
    知母皂苷B; Anemarsaponin B CFN99532 139051-27-7 C45H74O18 = 903.04 20mg QQ客服:2932563308
    知母皂苷BIII; Anemarsaponin BIII CFN90778 142759-74-8 C45H74O18 = 903.1 5mg QQ客服:215959384
    知母皂苷C; Timorsaponin C CFN90779 185432-00-2 C45H74O18 = 903.1 5mg QQ客服:2159513211
    伪原皂苷Pa; Parisyunnanoside B CFN90704 945865-37-2 C50H80O21 = 1016.0 5mg QQ客服:3257982914
    伪原薯蓣皂苷; Pseudoprotodioscin CFN90694 102115-79-7 C51H82O2 = 1031.18 20mg QQ客服:1148253675
    蒺藜皂苷K; Terrestrosin K CFN90822 193605-07-1 C51H82O24 = 1079.2 10mg QQ客服:1413575084
    伪原皂苷Pb; Pseudoproto Pb CFN90705 102100-46-9 C57H92O25 = 1177.4 5mg QQ客服:1413575084
    新知母皂苷BII; Officinalisinin I CFN90692 57944-18-0 C45H76O19 = 921.07 20mg QQ客服:2159513211
    知母皂苷E; Anemarsaponin E CFN99533 136565-73-6 C46H78O19 = 934.03 10mg QQ客服:1148253675

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