This study aims to investigate whether tested organic extracts possess antithrombotic properties with minimal or no toxicity and to predict the activity of some of their isolated compounds.
METHODS AND RESULTS:
An in vitro thrombolytic model was used to check the clot lysis effect of four Bangladeshi herbal extracts viz., roots ofCurculigo recurvataW.T. Aiton (Satipata), leaf ofAmorphophallus bulbiferRoxb. (Olkachu), leaf ofPhyllanthus sikkimensisMuell. Arg., and whole plant ofThunbergia grandifloraRoxb. (Nillata) using streptokinase as a positive control and water as a negative control. Cytotoxicity was screened by brine shrimp lethality bioassay using vincristine sulfate as positive control. In silico prediction of activity spectra for substances (PASS) prediction was applied for phytoconstituents, namely, Nyasicoside, glucomannan, grandifloric acid, serine, and alanine. Using an in vitro thrombolytic model,C. recurvata,A. bulbifer,P. sikkimensis, andT. grandiflorashowed 28.10±1.64%, 42.47±1.96%, 32.86±1.92%, and 25.51±1.67% of clot lysis, respectively. Reference drug streptokinase exhibited 75.00±3.04% clot lysis. Examined herbs showed significant (p<0.001) percentage (%) of clot lysis compared to negative control. In brine shrimp cytotoxic assay,C. recurvata,A. bulbifer,P. sikkimensis, andT. grandiflorashowed LC50values 210.64±3.44, 98.51±1.47, 187.29±2.01, and 386.43±3.02 μg/mL, respectively, with reference to vincristine sulfate (LC500.76±0.04). PASS predicted that examined phytoconstituents have a wide range of biological activity.
CONCLUSIONS:
Through our study it was found thatA. bulbiferandP. sikkimensiscould be considered as very promising and beneficial thrombolytic agents. |
Cell. 2018 Jan 11;172(1-2):249-261.e12. doi: 10.1016/j.cell.2017.12.019.IF=36.216(2019)
Cell Metab. 2020 Mar 3;31(3):534-548.e5. doi: 10.1016/j.cmet.2020.01.002.IF=22.415(2019)
Mol Cell. 2017 Nov 16;68(4):673-685.e6. doi: 10.1016/j.molcel.2017.10.022.IF=14.548(2019)
ACS Nano. 2018 Apr 24;12(4): 3385-3396. doi: 10.1021/acsnano.7b08969.IF=13.903(2019)
Nature Plants. 2016 Dec 22;3: 16206. doi: 10.1038/nplants.2016.205.IF=13.297(2019)
Sci Adv. 2018 Oct 24;4(10): eaat6994. doi: 10.1126/sciadv.aat6994.IF=12.804(2019)
