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  • L-苯丙氨酸

    L-Phenylalanine

    L-苯丙氨酸
    产品编号 CFN93152
    CAS编号 63-91-2
    分子式 = 分子量 C9H11NO2 = 165.19
    产品纯度 >=98%
    物理属性 Powder
    化合物类型 Alkaloids
    植物来源 The seeds of Glycine max (L.) Merr
    ChemFaces的产品在影响因子大于5的优秀和顶级科学期刊中被引用
    提供自定义包装
    产品名称 产品编号 CAS编号 包装 QQ客服
    L-苯丙氨酸 CFN93152 63-91-2 10mg QQ客服:215959384
    L-苯丙氨酸 CFN93152 63-91-2 20mg QQ客服:215959384
    L-苯丙氨酸 CFN93152 63-91-2 50mg QQ客服:215959384
    L-苯丙氨酸 CFN93152 63-91-2 100mg QQ客服:215959384
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    ChemFaces的产品在许多优秀和顶级科学期刊中被引用

    Cell. 2018 Jan 11;172(1-2):249-261.e12.
    doi: 10.1016/j.cell.2017.12.019.
    IF=36.216(2019)

    PMID: 29328914

    Cell Metab. 2020 Mar 3;31(3):534-548.e5.
    doi: 10.1016/j.cmet.2020.01.002.
    IF=22.415(2019)

    PMID: 32004475

    Mol Cell. 2017 Nov 16;68(4):673-685.e6.
    doi: 10.1016/j.molcel.2017.10.022.
    IF=14.548(2019)

    PMID: 29149595

    ACS Nano. 2018 Apr 24;12(4): 3385-3396.
    doi: 10.1021/acsnano.7b08969.
    IF=13.903(2019)

    PMID: 29553709

    Nature Plants. 2016 Dec 22;3: 16206.
    doi: 10.1038/nplants.2016.205.
    IF=13.297(2019)

    PMID: 28005066

    Sci Adv. 2018 Oct 24;4(10): eaat6994.
    doi: 10.1126/sciadv.aat6994.
    IF=12.804(2019)

    PMID: 30417089
    我们的产品现已经出口到下面的研究机构与大学,并且还在增涨
  • Universitas Airlangga (Indonesia)
  • Research Unit Molecular Epigenetics (MEG) (Germany)
  • Julius Kühn-Institut (Germany)
  • Universidad Miguel Hernández (Spain)
  • Northeast Normal University Changchun (China)
  • Sanford Burnham Medical Research Institute (USA)
  • Lund University (Sweden)
  • Universidade Federal de Santa Catarina (Brazil)
  • University of Malaya (Malaysia)
  • Shanghai University of TCM (China)
  • Uniwersytet Jagielloński w Krakowie (Poland)
  • Texas A&M University (USA)
  • University of Cincinnati (USA)
  • Universidade Federal de Goias (UFG) (Brazil)
  • More...
  • 国外学术期刊发表的引用ChemFaces产品的部分文献
  • Food and Bioprocess Technology2017, 10(6):1074-1092
  • Molecules.2020, 25(18),4089.
  • Korean J of Medicinal Crop Science2018, 220-226
  • Antioxidants (Basel).2023, 12(12):2078.
  • J Nat Prod.2017, 80(4):854-863
  • J Ethnopharmacol.2023, 321:117501.
  • Evid Based Complement Alternat Med.2018, 2018:8565132
  • Biocell2023, 47(8):1793-1802
  • PLoS One.2021, 16(9):e0257243.
  • Journal of Ginseng Research2021, 25 November
  • J Biol Chem.2014, 289(3):1723-31
  • Pharmaceuticals (Basel).2024, 17(4):442.
  • Front Pharmacol.2021, 12:635510.
  • Srinagarind Medical Journal2019, 34(1)
  • Applied Biological Chem. 2020, 26(63).
  • Research J. Pharm. and Tech.2020, 13(7):3059-3064.
  • Phytother Res.2023, 37(10):4587-4606.
  • Biomolecules.2020, 10(2):E184
  • J Agric Food Chem.2020, 68(43):12164-12172.
  • Phytother Res.2019, 33(7):1784-1793
  • J Sci Food Agric.2017, 97(5):1656-1662
  • Sci Rep.2019, 9(1):6429
  • Comp. & Mathematical Methods in Med.2022, 5475559.
  • ...
  • 生物活性
    Description: L-Phenylalanine has antibacterial activity.
    Targets: Antifection
    In vitro:
    Biomed Khim. 2014 Jul-Aug;60(4):448-61.
    Microbial synthesis of deuterium labelled L-phenylalanine with different levels of isotopic enrichment by facultative methylotrophic bacterium Brevibacterium methylicum with RMP assimilation of carbon.[Pubmed: 25249528]

    METHODS AND RESULTS:
    The preparative microbial synthesis of amino acids labelled with stable isotopes, including deuterium ( 2 H), suitable for biomedical applications by methylotrophic bacteria was studied using L-Phenylalanine as example. This amino acid is secreted by Gram-negative aerobic facultative methylotrophic bacteria Brevibacterium methylicum, assimilating methanol via ribulose-5-monophosphate (RMP) cycle of assimilation of carbon, The data on adaptation of L-Phenylalanine secreted by methylotrophic bacterium В. methylicum to the maximal concentration of deuterium in the growth medium with 98% 2 Н 2 O and 2% [ 2 Н]methanol, and biosynthesis of deuterium labelled L-Phenylalanine With different levels of enrichment are presented. The strain was adapted by means of plating initial cells on firm (2% agarose) minimal growth media with an increasing gradient of 2 Н 2 O concentration from 0; 24.5; 49.0; 73.5 up to 98% 2 Н 2 O followed by subsequent selection of separate colonies stable to the action of 2 Н 2 O. These colonies were capable to produce L-Phenylalanine. L-Phenylalanine was extracted from growth medium by extraction with isopropanol with the subsequent crystallization in ethanol (output 0.65 g/l).
    CONCLUSIONS:
    The developed method of microbial synthesis allows to obtain deuterium labelled L-Phenylalanine with different levels of isotopic enrichment, depending on concentration of 2 Н 2 O in growth media, from 17% (on growth medium with 24,5% 2 Н 2 O) up to 75% (on growth medium with 98% 2 Н 2 O) of deuterium in the molecule that is confirmed with the data of the electron impact (EI) mass- spectrometry analysis of methyl ethers of N-dimethylamino(naphthalene)-5-sulfochloride (dansyl) phenylalanine in these experimental conditions.
    Biotechnol Appl Biochem . 2018 May;65(3):476-483.
    Enhancement of l-phenylalanine production in Escherichia coli by heterologous expression of Vitreoscilla hemoglobin[Pubmed: 28872702]
    Abstract l-Phenylalanine is an important amino acid that is widely used in the production of food flavors and pharmaceuticals. Generally, l-phenylalanine production by engineered Escherichia coli requires a high rate of oxygen supply. However, the coexpression of Vitreoscilla hemoglobin gene (vgb), driven bya tac promoter, with the genes encoding 3-deoxy-d-arabinoheptulosonate-7-phosphate synthetase (aroF) and feedback-resistant chorismate mutase/prephenate dehydratase (pheAfbr ), led to increased productivity and decreased demand for aeration by E. coli CICC10245. Shake-flask studies showed that vgb-expressing strains displayed higher rates of oxygen uptake, and l-phenylalanine production under standard aeration conditions was increased. In the aerobic fermentation process, cell growth, l-phenylalanine production, and glucose consumption by the recombinant E. coli strain PAPV, which harbored aroF, pheAfbr , and tac-vgb genes, were increased compared to that in the strain harboring only aroF and pheAfbr (E. coli strain PAP), especially under oxygen-limited conditions. The vgb-expressing strain PAPV produced 21.9% more biomass and 16.6% more l-phenylalanine, while consuming only approximately 5% more glucose after 48 H of fermentation. This study demonstrates a method to enhance the l-phenylalanine production by E. coli using less intensive and thus more economical aeration conditions. Keywords: Escherichia coli; Vitreoscilla hemoglobin; dissolved oxygen; feedback resistant; l-phenylalanine; vgb.
    In vivo:
    Biotechnol Bioeng. 2014 Jul;111(7):1406-16.
    Improvement of constraint-based flux estimation during L-phenylalanine production with Escherichia coli using targeted knock-out mutants.[Pubmed: 24449451]

    METHODS AND RESULTS:
    Fed-batch production of the aromatic amino acid L-phenylalanine was studied with recombinant Escherichia coli strains on a 15 L-scale using glycerol as carbon source. Flux Variability Analysis (FVA) was applied for intracellular flux estimation to obtain an insight into intracellular flux distribution during L-phenylalanine production. Variability analysis revealed great flux uncertainties in the central carbon metabolism, especially concerning malate consumption. Due to these results two recombinant strains were genetically engineered differing in the ability of malate degradation and anaplerotic reactions (E. coli FUS4.11 ΔmaeA pF81kan and E. coli FUS4.11 ΔmaeA ΔmaeB pF81kan). Applying these malic enzyme knock-out mutants in the standardized L-phenylalanine production process resulted in almost identical process performances (e.g., L-phenylalanine concentration, production rate and byproduct formation). This clearly highlighted great redundancies in central metabolism in E. coli.
    CONCLUSIONS:
    Uncertainties of intracellular flux estimations by constraint-based analyses during fed-batch production of L-phenylalanine were drastically reduced by application of the malic enzyme knock-out mutants.
    制备储备液(仅供参考)
    1 mg 5 mg 10 mg 20 mg 25 mg
    1 mM 6.0536 mL 30.2682 mL 60.5364 mL 121.0727 mL 151.3409 mL
    5 mM 1.2107 mL 6.0536 mL 12.1073 mL 24.2145 mL 30.2682 mL
    10 mM 0.6054 mL 3.0268 mL 6.0536 mL 12.1073 mL 15.1341 mL
    50 mM 0.1211 mL 0.6054 mL 1.2107 mL 2.4215 mL 3.0268 mL
    100 mM 0.0605 mL 0.3027 mL 0.6054 mL 1.2107 mL 1.5134 mL
    * Note: If you are in the process of experiment, it's need to make the dilution ratios of the samples. The dilution data of the sheet for your reference. Normally, it's can get a better solubility within lower of Concentrations.
    部分图片展示
    产品名称 产品编号 CAS编号 分子式 = 分子量 位单 联系QQ
    1,2,3,19-四羟基-12-乌苏烯-28-酸; 1,2,3,19-Tetrahydroxy-12-ursen-28-oic acid CFN99233 113558-03-5 C30H48O6 = 504.7 5mg QQ客服:1413575084
    Triciribine; Triciribine CFN60328 35943-35-2 C13H16N6O4 = 320.3 5mg QQ客服:2159513211
    Crenolanib (CP-868596); Crenolanib (CP-868596) CFN60189 670220-88-9 C26H29N5O2 = 443.54 5mg QQ客服:215959384
    豆瓣菜苷; Gluconasturtiin CFN00489 18425-76-8 C15H20KNO9S2 = 461.6 5mg QQ客服:215959384

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