L-苯丙氨酸

L-Phenylalanine

	产品编号	CFN93152
	CAS编号	63-91-2
	分子式 = 分子量	C₉H₁₁NO₂ = 165.19
	产品纯度	>=98%
	物理属性	Powder
	化合物类型	Alkaloids
	植物来源	The seeds of Glycine max (L.) Merr
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产品名称	产品编号	CAS编号	包装	QQ客服
L-苯丙氨酸	CFN93152	63-91-2	10mg	QQ客服：2056216494
L-苯丙氨酸	CFN93152	63-91-2	20mg	QQ客服：2056216494
L-苯丙氨酸	CFN93152	63-91-2	50mg	QQ客服：2056216494
L-苯丙氨酸	CFN93152	63-91-2	100mg	QQ客服：2056216494

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Description:

L-Phenylalanine has antibacterial activity.

Targets:

Antifection

In vitro:

Biomed Khim. 2014 Jul-Aug;60(4):448-61.

Microbial synthesis of deuterium labelled L-phenylalanine with different levels of isotopic enrichment by facultative methylotrophic bacterium Brevibacterium methylicum with RMP assimilation of carbon.[Pubmed: 25249528]

METHODS AND RESULTS:
The preparative microbial synthesis of amino acids labelled with stable isotopes, including deuterium ( 2 H), suitable for biomedical applications by methylotrophic bacteria was studied using L-Phenylalanine as example. This amino acid is secreted by Gram-negative aerobic facultative methylotrophic bacteria Brevibacterium methylicum, assimilating methanol via ribulose-5-monophosphate (RMP) cycle of assimilation of carbon, The data on adaptation of L-Phenylalanine secreted by methylotrophic bacterium В. methylicum to the maximal concentration of deuterium in the growth medium with 98% 2 Н 2 O and 2% [ 2 Н]methanol, and biosynthesis of deuterium labelled L-Phenylalanine With different levels of enrichment are presented. The strain was adapted by means of plating initial cells on firm (2% agarose) minimal growth media with an increasing gradient of 2 Н 2 O concentration from 0; 24.5; 49.0; 73.5 up to 98% 2 Н 2 O followed by subsequent selection of separate colonies stable to the action of 2 Н 2 O. These colonies were capable to produce L-Phenylalanine. L-Phenylalanine was extracted from growth medium by extraction with isopropanol with the subsequent crystallization in ethanol (output 0.65 g/l).
CONCLUSIONS:
The developed method of microbial synthesis allows to obtain deuterium labelled L-Phenylalanine with different levels of isotopic enrichment, depending on concentration of 2 Н 2 O in growth media, from 17% (on growth medium with 24,5% 2 Н 2 O) up to 75% (on growth medium with 98% 2 Н 2 O) of deuterium in the molecule that is confirmed with the data of the electron impact (EI) mass- spectrometry analysis of methyl ethers of N-dimethylamino(naphthalene)-5-sulfochloride (dansyl) phenylalanine in these experimental conditions.

Biotechnol Appl Biochem . 2018 May;65(3):476-483.

Enhancement of l-phenylalanine production in Escherichia coli by heterologous expression of Vitreoscilla hemoglobin[Pubmed: 28872702]

Abstract l-Phenylalanine is an important amino acid that is widely used in the production of food flavors and pharmaceuticals. Generally, l-phenylalanine production by engineered Escherichia coli requires a high rate of oxygen supply. However, the coexpression of Vitreoscilla hemoglobin gene (vgb), driven bya tac promoter, with the genes encoding 3-deoxy-d-arabinoheptulosonate-7-phosphate synthetase (aroF) and feedback-resistant chorismate mutase/prephenate dehydratase (pheAfbr ), led to increased productivity and decreased demand for aeration by E. coli CICC10245. Shake-flask studies showed that vgb-expressing strains displayed higher rates of oxygen uptake, and l-phenylalanine production under standard aeration conditions was increased. In the aerobic fermentation process, cell growth, l-phenylalanine production, and glucose consumption by the recombinant E. coli strain PAPV, which harbored aroF, pheAfbr , and tac-vgb genes, were increased compared to that in the strain harboring only aroF and pheAfbr (E. coli strain PAP), especially under oxygen-limited conditions. The vgb-expressing strain PAPV produced 21.9% more biomass and 16.6% more l-phenylalanine, while consuming only approximately 5% more glucose after 48 H of fermentation. This study demonstrates a method to enhance the l-phenylalanine production by E. coli using less intensive and thus more economical aeration conditions. Keywords: Escherichia coli; Vitreoscilla hemoglobin; dissolved oxygen; feedback resistant; l-phenylalanine; vgb.

In vivo:

Biotechnol Bioeng. 2014 Jul;111(7):1406-16.

Improvement of constraint-based flux estimation during L-phenylalanine production with Escherichia coli using targeted knock-out mutants.[Pubmed: 24449451]

METHODS AND RESULTS:
Fed-batch production of the aromatic amino acid L-phenylalanine was studied with recombinant Escherichia coli strains on a 15 L-scale using glycerol as carbon source. Flux Variability Analysis (FVA) was applied for intracellular flux estimation to obtain an insight into intracellular flux distribution during L-phenylalanine production. Variability analysis revealed great flux uncertainties in the central carbon metabolism, especially concerning malate consumption. Due to these results two recombinant strains were genetically engineered differing in the ability of malate degradation and anaplerotic reactions (E. coli FUS4.11 ΔmaeA pF81kan and E. coli FUS4.11 ΔmaeA ΔmaeB pF81kan). Applying these malic enzyme knock-out mutants in the standardized L-phenylalanine production process resulted in almost identical process performances (e.g., L-phenylalanine concentration, production rate and byproduct formation). This clearly highlighted great redundancies in central metabolism in E. coli.
CONCLUSIONS:
Uncertainties of intracellular flux estimations by constraint-based analyses during fed-batch production of L-phenylalanine were drastically reduced by application of the malic enzyme knock-out mutants.

	1 mg	5 mg	10 mg	20 mg	25 mg
1 mM	6.0536 mL	30.2682 mL	60.5364 mL	121.0727 mL	151.3409 mL
5 mM	1.2107 mL	6.0536 mL	12.1073 mL	24.2145 mL	30.2682 mL
10 mM	0.6054 mL	3.0268 mL	6.0536 mL	12.1073 mL	15.1341 mL
50 mM	0.1211 mL	0.6054 mL	1.2107 mL	2.4215 mL	3.0268 mL
100 mM	0.0605 mL	0.3027 mL	0.6054 mL	1.2107 mL	1.5134 mL

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L-苯丙氨酸

L-Phenylalanine

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