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  • 防己诺林碱; 粉防己乙素; 汉防己乙素; 去甲粉防己碱

    Fangchinoline

    防己诺林碱; 粉防己乙素; 汉防己乙素; 去甲粉防己碱
    产品编号 CFN99167
    CAS编号 436-77-1
    分子式 = 分子量 C37H40N2O6 = 608.71
    产品纯度 >=98%
    物理属性 Powder
    化合物类型 Alkaloids
    植物来源 The vines of Cocculus orbiculatus (L.) DC.
    ChemFaces的产品在影响因子大于5的优秀和顶级科学期刊中被引用
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    防己诺林碱; 粉防己乙素; 汉防己乙素; 去甲粉防己碱 CFN99167 436-77-1 10mg QQ客服:1413575084
    防己诺林碱; 粉防己乙素; 汉防己乙素; 去甲粉防己碱 CFN99167 436-77-1 20mg QQ客服:1413575084
    防己诺林碱; 粉防己乙素; 汉防己乙素; 去甲粉防己碱 CFN99167 436-77-1 50mg QQ客服:1413575084
    防己诺林碱; 粉防己乙素; 汉防己乙素; 去甲粉防己碱 CFN99167 436-77-1 100mg QQ客服:1413575084
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    ChemFaces的产品在许多优秀和顶级科学期刊中被引用

    Cell. 2018 Jan 11;172(1-2):249-261.e12.
    doi: 10.1016/j.cell.2017.12.019.
    IF=36.216(2019)

    PMID: 29328914

    Cell Metab. 2020 Mar 3;31(3):534-548.e5.
    doi: 10.1016/j.cmet.2020.01.002.
    IF=22.415(2019)

    PMID: 32004475

    Mol Cell. 2017 Nov 16;68(4):673-685.e6.
    doi: 10.1016/j.molcel.2017.10.022.
    IF=14.548(2019)

    PMID: 29149595

    ACS Nano. 2018 Apr 24;12(4): 3385-3396.
    doi: 10.1021/acsnano.7b08969.
    IF=13.903(2019)

    PMID: 29553709

    Nature Plants. 2016 Dec 22;3: 16206.
    doi: 10.1038/nplants.2016.205.
    IF=13.297(2019)

    PMID: 28005066

    Sci Adv. 2018 Oct 24;4(10): eaat6994.
    doi: 10.1126/sciadv.aat6994.
    IF=12.804(2019)

    PMID: 30417089
    我们的产品现已经出口到下面的研究机构与大学,并且还在增涨
  • Centrum Menselijke Erfelijkheid (Belgium)
  • Kitasato University (Japan)
  • Molecular Biology Institute of Barcelona (IBMB)-CSIC (Spain)
  • Tokyo Woman's Christian University (Japan)
  • Anna University (India)
  • Chang Gung University (Taiwan)
  • National Hellenic Research Foundation (Greece)
  • Universidad de Antioquia (Colombia)
  • The Ohio State University (USA)
  • The Vancouver Prostate Centre (VPC) (Canada)
  • Uniwersytet Jagielloński w Krakowie (Poland)
  • National Cancer Center Research Institute (Japan)
  • Institute of Tropical Disease Universitas Airlangga (Indonesia)
  • Universidade do Porto (Portugal)
  • More...
  • 国外学术期刊发表的引用ChemFaces产品的部分文献
  • J Chromatogr B Analyt Technol Biomed Life Sci.2019, 1113:1-13
  • Korean Herb. Med. Inf.2021, 9(2):231-239.
  • Int J Mol Sci.2019, 20(8):E1855
  • Exp Mol Med.2020, 52(4):629-642.
  • Nutrients.2019, 12(1):E40
  • Journal of Chromatography A2020, 460942
  • Molecules.2019, 24(1):E159
  • Lab Chip.2018, 18(6):971-978
  • Chem Biol Interact.2018, 283:59-74
  • Food Sci Biotechnol.2016, 25(5):1437-1442
  • Cells.2023, 12(1):168.
  • Nutrients.2019, 11(11):E2694
  • Nutrients.2019, 11(4):E936
  • J Ethnopharmacol.2020, 269:113752.
  • Tumour Biol.2015, 36(9):7027-34
  • Anticancer Res.2014, 34(7):3505-9
  • Int J Biol Macromol.2020, 169:342-351
  • Agronomy 2021, 11(3),502.
  • Cardiovasc Toxicol.2019, 19(4):297-305
  • Genes Genomics.2020, 10.1007
  • Microchemical Journal2018, 137:168-173
  • J Sep Sci.2020, 43(22):4148-4161.
  • bioRxiv-Pharm.&Toxi.2022, 2022.481203.
  • ...
  • 生物活性
    Description: Fangchinoline is a nonspecific Ca2+ channel blocker, which has neuroprotective, antioxidant, anti-inflammatory, anti-cancer, vasodilating and hypotensive effects. It inhibited cell proliferation via Akt/GSK-3beta/ cyclin D1 signaling and induceed apoptosis in MDA-MB-231 breast cancer cells. It can inhibit human immunodeficiency virus type 1 replication by interfering with gp160 proteolytic processing.
    Targets: FAK | MMP(e.g.TIMP) | Akt | MEK | ERK | GSK-3 | Bcl-2/Bax | HIV | CDK | p21 | Calcium Channel | MAPK | IL Receptor
    In vitro:
    PLoS One. 2012;7(6):e39225.
    Fangchinoline inhibits human immunodeficiency virus type 1 replication by interfering with gp160 proteolytic processing.[Pubmed: 22720080]
    The introduction of highly active antiretroviral therapy has led to a significant reduction in the morbidity and mortality of acquired immunodeficiency syndrome patients. However, the emergence of drug resistance has resulted in the failure of treatments in large numbers of patients and thus necessitates the development of new classes of anti-HIV drugs.
    METHODS AND RESULTS:
    In this study, more than 200 plant-derived small-molecule compounds were evaluated in a cell-based HIV-1 antiviral screen, resulting in the identification of a novel HIV-1 inhibitor (fangchinoline). Fangchinoline, a bisbenzylisoquinoline alkaloid isolated from Radix Stephaniae tetrandrae, exhibited antiviral activity against HIV-1 laboratory strains NL4-3, LAI and BaL in MT-4 and PM1 cells with a 50% effective concentration ranging from 0.8 to 1.7 μM. Mechanism-of-action studies showed that fangchinoline did not exhibit measurable antiviral activity in TZM-b1 cells but did inhibit the production of infectious virions in HIV-1 cDNA transfected 293T cells, which suggests that the compound targets a late event in infection cycle. Furthermore, the antiviral effect of fangchinoline seems to be HIV-1 envelope-dependent, as the production of infectious HIV-1 particles packaged with a heterologous envelope, the vesicular stomatitis virus G glycoprotein, was unaffected by fangchinoline. Western blot analysis of HIV envelope proteins expressed in transfected 293T cells and in isolated virions showed that fangchinoline inhibited HIV-1 gp160 processing, resulting in reduced envelope glycoprotein incorporation into nascent virions.
    CONCLUSIONS:
    Collectively, our results demonstrate that fangchinoline inhibits HIV-1 replication by interfering with gp160 proteolytic processing. Fangchinoline may serve as a starting point for developing a new HIV-1 therapeutic approach.
    Biochem Pharmacol. 2003 Nov 1;66(9):1853-60.
    Fangchinoline inhibits rat aortic vascular smooth muscle cell proliferation and cell cycle progression through inhibition of ERK1/2 activation and c-fos expression.[Pubmed: 14563495]
    Fangchinoline (FAN; a plant alkaloid isolated from Stephania tetrandrae) is a nonspecific Ca(2+) channel blocker. The objective of the present study was to investigate the effect of FAN on the growth factor-induced proliferation of primary cultured rat aortic smooth muscle cells (RASMCs).
    METHODS AND RESULTS:
    FAN significantly inhibited both 5% fetal bovine serum (FBS)- and 50ng/mL platelet-derived growth factor (PDGF)-BB-induced proliferation, [3H]thymidine incorporation into DNA and phosphorylation of extracellular signal-regulated kinase 1/2. In accordance with these findings, FAN revealed blocking of the FBS-inducible progression through G(0)/G(1) to S phase of the cell cycle in synchronized cells and caused a 62% decrease in the early elevation of c-fos expression induced after 5% FBS addition. Furthermore, significant antiproliferative activity of FAN is observed at concentrations below those required to achieve significant inhibition of Ca(2+) channels by FAN. These results suggest that FAN reduced both FBS- and PDGF-BB-induced RASMCs proliferation by perturbing cell cycle progression.
    CONCLUSIONS:
    This antiproliferative effect of FAN is dependent on the MAP kinase pathway, but cannot be limited to its Ca(2+) modulation.
    J Ethnopharmacol. 2000 Feb;69(2):173-9.
    Anti-inflammatory effects of fangchinoline and tetrandrine.[Pubmed: 10687873]
    Fangchinoline and tetrandrine are the major alkaloids from Stephania tetrandrae S. Moore which has been used traditionally for the treatment of inflammatory diseases in oriental countries including Korea.
    METHODS AND RESULTS:
    Both fangchinoline and tetrandrine showed anti-inflammatory effects on mouse ear edema induced by croton oil. In addition, the effects of fangchinoline and tetrandrine on cyclooxygenase, murine interleukin-5 (mIL-5) and human interleukin-6 (hIL-6) were examined in vitro to investigate the anti-inflammatory action mechanisms. One hundred micromolar of fangchinoline showed 35% of inhibition on cyclooxygenase, but the same concentration of tetrandrine did not show any inhibition. On the other hand, 12.5 microM of tetrandrine exhibited 95% of inhibition on mIL-5 activity, while fangchinoline did not show any effects. However, 4 microM of fangchinoline and 6 microM of tetrandrine showed 63 and 86% of inhibitions on hIL-6 activity, respectively.
    CONCLUSIONS:
    These results suggest that biochemical mechanisms of fangchinoline and tetrandrine on anti-inflammation are significantly different even though they are similar in chemical structure.
    Cell Physiol Biochem . 2017;43(3):1003-1011.
    Fangchinoline Induces Apoptosis, Autophagy and Energetic Impairment in Bladder Cancer[Pubmed: 28968601]
    Abstract Background/aims: Tetrandrine and Fangchinoline (Fcn) are two natural products that are found in Stephania tetrandra. Tetrandrine is a known anti-bladder cancer compound, but the effects of Fcn on bladder cancer have been previously unclear. In the present study, we focused on the anti-tumor effects of Fcn on bladder cancer. Methods and results: We treated T24 and 5637 bladder cancer cell lines with Fcn in vitro. We observed that Fcn inhibited the viability of bladder cancer cells in a concentration-dependent manner. The expression of PCNA, a biomarker of proliferation, was down-regulated. Fcn treatment induced both apoptosis and autophagy in bladder cancer cells, as shown by the increased cleavage of caspase-3, an up-regulated LC3-II/LC3-I ratio and the down-regulated p62 level. Blocking autophagy with 3-MA (3-Methyladenine) enhanced Fcn-induced apoptosis, indicating that Fcn-induced autophagy was adaptive. Additionally, we observed that Fcn treatment inhibited mTOR and reduced the intracellular ATP levels. The exogenous addition of methyl pyruvate (MP) to compensate metabolic substrates alleviated Fcn-induced apoptosis and autophagy. Conclusions: Our data indicated that Fcn caused an impairment in energy generation, which led to apoptosis and adaptive autophagy in bladder cancer. These results demonstrated that Fcn may be a potential candidate for use in the prevention and treatment of bladder cancer. Keywords: Apoptosis; Autophagy; Bladder cancer; Fangchinoline; Proliferation.
    J Drug Target . 2015 Apr;23(3):266-74.
    Fangchinoline as a kinase inhibitor targets FAK and suppresses FAK-mediated signaling pathway in A549[Pubmed: 25539072]
    Abstract Background: Fangchinoline as a novel anti-tumor agent has been paid attention in several types of cancers cells except lung cancer. Here we have investigated the effect of fangchinoline on A549 cells and its underlying mechanism. Purpose: The purpose of this work was to study the effect of fangchinoline on A549 cells. Methods: Four lung cancer cell lines (A549, NCI-H292, NCI-H446, and NCI-H460) were exposed to varying concentrations (10-40 μmol/l) of fangchinoline to observe the effect of fangchinoline on the four lung cancer cell lines and to observe the changes of the lung cancer cell on proliferation, apoptosis, and invasion. Results: Fangchinoline effectively suppressed proliferation and invasion of A549 cell line but not NCI-H292, NCI-H446, and NCI-H460 cell lines by inhibiting the phosphorylation of FAK (Tyr397) and its downstream pathways, due to the significant differences of Fak expression between A549 and the other three cell lines. And all FAK-paxillin/MMP2/MMP9 pathway, FAK-Akt pathway, and FAK-MEK-ERK1/2 pathway could be inhibited by fangchinoline. Discussion: Fangchinoline effectively suppressed proliferation and invasion of A549 cell line by inhibiting the phosphorylation of FAK (Tyr397) and its downstream pathways. Conclusion: Fangchinoline could inhibit the phosphorylation of FAK(p-Tyr397), at least partially. Fangchinoline as a kinase inhibitor targets FAK and suppresses FAK-mediated signaling pathway and inhibits the growth and the invasion in tumor cells which highly expressed FAK such as A549 cell line. Keywords: FAK; fangchinoline; lung cancer cell; phosphorylation; signaling pathway.
    In vivo:
    J Ethnopharmacol. 1997 Oct;58(2):117-23.
    Vasodilating and hypotensive effects of fangchinoline and tetrandrine on the rat aorta and the stroke-prone spontaneously hypertensive rat.[Pubmed: 9406900]
    Comparative studies of the effects of tetrandrine (TET) and fangchinoline (FAN), two major components of the Radix of Stephannia tetrandrae, on vasodilations and on calcium movement in vascular smooth muscle, and studies of hypotensive effects on stroke-prone spontaneously hypertensive rats (SHRSP) were performed in the following experiments.
    METHODS AND RESULTS:
    TET and FAN inhibited high K+ (65.4 mM) and induced sustained contraction in the rat aorta smooth muscle strips. IC50 values for TET and FAN were 0.27 +/- 0.05 microM (n = 6) and 9.53 +/- 1.57 microM (n = 6), respectively, and this inhibition was antagonized by increasing the Ca2+ concentration in the medium. The IC50 of TET for norepinephrine (NE)-induced contraction (0.86 +/- 0.04 g) was 3.08 +/- 0.05 microM (n = 4), and the IC50 of FAN for NE-induced contraction (0.88 +/- 0.07 g) was 14.20 +/- 0.40 microM (n = 4). At the molecular level, radiolabelled 45Ca2+ uptake tests revealed that TET and FAN also inhibited high K+ (65.4 mM) and 1 microM NE-stimulated Ca2+ influx in rat aorta strips at the maximal concentration was needed to inhibit the contraction. TET (3 mg/kg) and FAN (30 mg/kg) administered by intravenous (i.v.) bolus injection also lowered the mean arterial pressure (MAP) significantly during the period of observation in conscious SHRSP, respectively.
    CONCLUSIONS:
    These results showed that TET was more potent than FAN in blocking calcium channels and antihypertensive activity.
    制备储备液(仅供参考)
    1 mg 5 mg 10 mg 20 mg 25 mg
    1 mM 1.6428 mL 8.2141 mL 16.4282 mL 32.8564 mL 41.0705 mL
    5 mM 0.3286 mL 1.6428 mL 3.2856 mL 6.5713 mL 8.2141 mL
    10 mM 0.1643 mL 0.8214 mL 1.6428 mL 3.2856 mL 4.107 mL
    50 mM 0.0329 mL 0.1643 mL 0.3286 mL 0.6571 mL 0.8214 mL
    100 mM 0.0164 mL 0.0821 mL 0.1643 mL 0.3286 mL 0.4107 mL
    * Note: If you are in the process of experiment, it's need to make the dilution ratios of the samples. The dilution data of the sheet for your reference. Normally, it's can get a better solubility within lower of Concentrations.
    部分图片展示
    产品名称 产品编号 CAS编号 分子式 = 分子量 位单 联系QQ
    小檗胺; Berbamine CFN98727 478-61-5 C37H40N2O6 = 608.7 20mg QQ客服:1413575084
    汉防己甲素; 粉防己碱; Tetrandrine CFN99166 518-34-3 C38H42N2O6 = 622.76 20mg QQ客服:2056216494
    芬氏唐松草碱; Thalidezine CFN89488 18251-36-0 C38H42N2O7 = 638.74 5mg QQ客服:215959384
    鹤氏唐松草碱; Hernandezine CFN89519 6681-13-6 C39H44N2O7 = 652.77 5mg QQ客服:1457312923
    防己诺林碱; 粉防己乙素; 汉防己乙素; 去甲粉防己碱; Fangchinoline CFN99167 436-77-1 C37H40N2O6 = 608.71 20mg QQ客服:3257982914
    (-)-箭毒素; (-)-Curine CFN90532 436-05-5 C36H38N2O6 = 594.69 5mg QQ客服:215959384
    汉防己乙素; Demethyl tetrandrine CFN90482 33889-68-8 C37H40N2O6 = 608.72 20mg QQ客服:2159513211
    Thalrugosidine; Thalrugosidine CFN89510 33954-34-6 C38H42N2O7 = 638.74 5mg QQ客服:1413575084
    异粉防己碱N-2'-氧化物; Isotetrandrine N-2'-oxide CFN97190 70191-83-2 C38H42N2O7 = 638.8 5mg QQ客服:3257982914
    头花千金藤醇灵碱; Cepharanoline CFN91640 27686-34-6 C36H36N2O6 = 592.68 5mg QQ客服:3257982914

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