| In vitro: |
| Planta medica, 1994, 60(05):410-413. | | Chamazulene: An Antioxidant-Type Inhibitor of Leukotriene B4 Formation.[Reference: WebLink] | Matricine and its transformation product chamazulene are constituents of chamomile extracts. Both have been demonstrated to exert anti-inflammatory activity in vivo. Since preparations from chamomile are used for the treatment of inflammatory skin and bowel diseases, we studied the effects of these compounds on the leukotriene production in neutrophilic granulocytes.
METHODS AND RESULTS:
Chamazulene inhibited the formation of leukotriene B4 in intact cells and in the 105,000 × g supernatant fraction in a concentration-dependent manner. The IC50 values were 15 and 10 µM, respectively. Matricine showed no effect up to 200 µM. Chamazulene (IC50: 2 µM), but not matricine, blocked the chemical peroxidation of arachidonic acid. Additionally, matricine (up to 200 µM) had no effects on the cyclooxygenase and 12-lipoxygenase activities in human platelets.
CONCLUSIONS:
Therefore, it is concluded that chamazulene, but not matricine, may contribute to the anti-inflammatory activity of chamomile extracts by inhibiting the leukotriene synthesis and additional antioxidative effects. | | Research Communications in Molecular Pathology & Pharmacology, 1996, 92(3):361-364. | | Investigation of the effect of chamazulene on lipid peroxidation and free radical processes.[Reference: WebLink] | Oxygen toxicity and related free radical reactions are implicated in numerous pathophysiological conditions, like atherosclerosis, inflammation, gastric ulceration, neuronal degeneration, tumour promotion. The flowers of Matricaria chamomilla, Asteraceae, have been used therapeutically for conditions in which oxidative stress is supposed to be implicated.
METHODS AND RESULTS:
We considered interesting to investigate the effect of Chamazulene, the active substance of chamomile, on free radical processes. Membrane lipid peroxidation was induced by Fe2+/ascorbate and assessed as the 2-thiobarbituric acid reactive material. The hydroxyl radical scavenging activity was studied as the competition of Chamazulene with DMSO for HO. generated by Fe3+/ascorbate. Finally, the interaction of Chamazulene with the N-centered stable free radical DPPH was estimated photometrically (517 nm). It was found that Chamazulene inhibited lipid peroxidation in a concentration and time dependent manner presenting an IC50 of 18 microM after 45 min incubation. It could also inhibit the autoxidation of DMSO (33 mM) by 76% at 25 mM, and had a weak capacity to interact with DPPH.
CONCLUSIONS:
In conclusion, Chamazulene presents interesting properties concerning radical processes. | | Natural Product Research,2014, 28(24):2321-2323. | | Antioxidant and radical scavenging activities of chamazulene.[Reference: WebLink] | Essential oils (EOs) of chamomile contain several bioactive compounds, including monoterpenes, sesquiterpenes, triterpenes and fatty acids. Hydrodistillation of chamomile EO induces the formation of chamazulene, a bioactive compound.
METHODS AND RESULTS:
Chamazulene was isolated from the EO by column chromatography. The total antioxidant capacity confirmed a higher antioxidant activity of chamazulene (IC50 = 6.4 μg mL(- 1)) than of ascorbic acid (IC50 = 12.8 μg mL(- 1)), α-tocopherol (IC50 = 20.5 μg mL(- 1)) and of butylated hydroxytoluene (BHT) (IC50 = 30.8 μg mL(- 1)). Chamazulene was unable to react with DPPH√. However, when chamazulene was assayed with ABTS√, a strong and significantly (P < 0.05) higher free radical scavenging activity was observed (IC50 = 3.7 μg mL(- 1)), with respect to BHT (IC50 = 6.2 μg mL(- 1)) and α-tocopherol (IC50 = 11.5 μg mL(- 1)).
CONCLUSIONS:
The results of this work show that chamazulene is an important factor for the antioxidant power of chamomile oil.
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