| In vitro: |
| 한국생물공학회 학술대회, 2008, 107(3):169-170. | | Secondary Metabolites Production in Angelica gigas Cell Culture by Two-Stage Process.[Reference: WebLink] | Angelica gigas is a common traditional medicinal plant that has been used for more than several thousand years in Korea. The commonly used Angelica roots showed pharmacological properties with their chemical compositions. The main chemical constituents of Angelica are decursin, decursinol angelate, Angelicide, brefeldin A, succinic acid and adenine(2). Main constituents associated with the pharmacological activities of Angelica roots had anticancer activity(1), inhibitor of platelet aggregation, antiasthmatic and spasmolytic activities(2). The economic feasibility of production by plant cell culture is primarily determined by productivity. It has been argued that optimization of biomass growth and product formation must be evaluated as to the possibility of implementation in a large-scale process(3). One of solutions was a two-stage process.
METHODS AND RESULTS:
We have changed hormone condition of Angelica gigas cell culture for two-stage culture. After analyzing intracellular compounds of our Angelica gigas cell line, we have validated to increase several secondary metabolites after changing culture medium.
CONCLUSIONS:
In this study, we suggest that two-stage process of plant cell culture is a good system for secondary metabolites production. |
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