| In vitro: |
| Anti-tumor Pharmacy, 2011,1(1) :30-2. | | Inhibitory action of nitidine and zanthobungeanine on lung adenocarcinoma A549 cell[Reference: WebLink] | To determine the inhibition of nitidine and Zanthobungeanine on the lung adenocarcinoma A549 cell.
METHODS AND RESULTS:
The IC50 of nitidine and Zanthobungeanine were evaluated by MTT photocolorimetric method and the apoptosis was evaluated by inverted microscope. The IC_(50) of nitidine was 0.68μg·mL~(-1) and the IC_(50) of Zanthobungeanine was 173.41μg·mL~(-1).The nitidine 1.015μg·mL~(-1) induced apoptosis of 66%of lung adenocarcinoma A549 cells.The typical apoptic morphological changes,including cell shrinkage,nuclear condensation,and nuclear fragment was observed under light microscope.
CONCLUSIONS:
Nitidine can kill the external A549 cell and inhibit the development of A549 cell,but Zanthobungeanine showed the inhibitory action only in high concentration and had no antipersonnel effect on A549 cell.Nitidine can inhibit the proliferation of lung adenocarcinoma A549 cell by inducing apoptosis.Zanthobungeanine didn't show such action. | | Journal of Shenyang Pharmaceutical University, 2013,30(2) :100-5. | | Isolation,identification and activity determination on the anti-inflammatory components of roots of Zanthoxylum nitidum[Reference: WebLink] | To study the chemical constituents of Zanthoxylum nitidum(Roxb.)DC.and find compounds with anti-inflammatory activity.
METHODS AND RESULTS:
Silica gel column chromatography,RP-ODS,Sephadex LH-20 and high performance liquid chromatography were applied for the isolation and purification of the constituents.The structures were elucidated by their physicochemical properties and spectral methods.,The inhibitory activity on NO production and cell survival was evaluated by MTT assay through measuring the absorbance using microplate reader. Thirteen compounds were isolated,including skimmianine(1),dictamnine(2),Zanthobungeanine(3),zanthodioline(4),N-methylflindersine(5),liriodenine(6),L-sesamin(7),β-amyrin(8),stigmast-9(11)-en-3-ol(9),tridecane amine(10),tetradecane amine(11),heptadecanoic amine(12)and nonadecane amine(13).
CONCLUSIONS:
Compounds 3,4,8,10,11,12 and 13 are all isolated from Zanthoxylum nitidum for the first time.Compound 3 shows moderate NO production inhibitory activity with an IC50 value of 37.26 mg · L-1. |
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